Mitochondrial DNA damage in calf skeletal muscle and walking performance in people with peripheral artery disease.
Ischemia-reperfusion
Oxidative stress
Peripheral artery disease (PAD)
mtDNA copy number
mtDNA deletion
mtDNA heteroplasmy
mtDNA mutations
Journal
Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159
Informations de publication
Date de publication:
20 11 2020
20 11 2020
Historique:
received:
29
06
2020
revised:
24
08
2020
accepted:
01
09
2020
pubmed:
11
9
2020
medline:
22
6
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
Peripheral artery disease (PAD) is associated with mitochondrial dysfunction in calf skeletal muscle and a greater abundance of mitochondrial DNA (mtDNA) heteroplasmy. However, it is unknown whether calf skeletal muscle mtDNA of PAD participants harbors a greater abundance of mitochondrial DNA 4977-bp common deletion (mtDNA Calf muscle biopsies were obtained from 50 PAD participants (ankle-brachial index (ABI) < 0.95) and 25 non-PAD participants (ABI = 0.99-1.40) matched by age, sex, and race. The abundance of mtDNA copy number, mtDNA Participants with PAD (mean age = 65.4 years, SD = 6.9; 14 (28%) women, 38 (76%) black) and without PAD (mean age = 65.2 years, SD = 6.7; 7 (28%) women, 16 (64%) black) did not differ in the abundance of calf muscle mtDNA Overall, these data suggest that the abundance of calf muscle mtDNA strand breaks and mtDNA
Sections du résumé
BACKGROUND
Peripheral artery disease (PAD) is associated with mitochondrial dysfunction in calf skeletal muscle and a greater abundance of mitochondrial DNA (mtDNA) heteroplasmy. However, it is unknown whether calf skeletal muscle mtDNA of PAD participants harbors a greater abundance of mitochondrial DNA 4977-bp common deletion (mtDNA
METHODS
Calf muscle biopsies were obtained from 50 PAD participants (ankle-brachial index (ABI) < 0.95) and 25 non-PAD participants (ABI = 0.99-1.40) matched by age, sex, and race. The abundance of mtDNA copy number, mtDNA
RESULTS
Participants with PAD (mean age = 65.4 years, SD = 6.9; 14 (28%) women, 38 (76%) black) and without PAD (mean age = 65.2 years, SD = 6.7; 7 (28%) women, 16 (64%) black) did not differ in the abundance of calf muscle mtDNA
CONCLUSION
Overall, these data suggest that the abundance of calf muscle mtDNA strand breaks and mtDNA
Identifiants
pubmed: 32911084
pii: S0891-5849(20)31241-7
doi: 10.1016/j.freeradbiomed.2020.09.004
pii:
doi:
Substances chimiques
DNA, Mitochondrial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
680-689Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.