Generation and characterization of human induced pluripotent stem cells lines from four patients diagnosed with schizophrenia and one healthy control.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
31
07
2020
revised:
17
08
2020
accepted:
21
08
2020
pubmed:
11
9
2020
medline:
29
5
2021
entrez:
10
9
2020
Statut:
ppublish
Résumé
Fibroblasts were isolated from skin biopsies of four patients diagnosed with schizophrenia and from one healthy control. Patient fibroblasts were transfected with five episomal, non-integrative reprogramming vectors to generate human induced pluripotent stem cells (iPSC). Reprogrammed iPSC showed consistent expression of several pluripotency markers, loss of expression of exogenous reprogramming vectors and ability to differentiate into all three germ layers. Additionally, iPSC maintained their normal karyotype during reprogramming. These generated cell lines can be used to study early neurodevelopmental and neuroinflammatory processes in schizophrenia in a patient-derived in vitro model.
Identifiants
pubmed: 32911325
pii: S1873-5061(20)30262-2
doi: 10.1016/j.scr.2020.101961
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101961Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.