Capacitation increases glucose consumption in murine sperm.
ATP
cAMP
capacitation
glucose consumption
glycolysis
sperm
Journal
Molecular reproduction and development
ISSN: 1098-2795
Titre abrégé: Mol Reprod Dev
Pays: United States
ID NLM: 8903333
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
22
08
2020
accepted:
28
08
2020
pubmed:
12
9
2020
medline:
27
8
2021
entrez:
11
9
2020
Statut:
ppublish
Résumé
Mammalian sperm acquire fertilization capacity in the female reproductive tract in a process known as capacitation. During capacitation, sperm change their motility pattern (i.e., hyperactivation) and become competent to undergo the acrosome reaction. We have recently shown that, in the mouse, sperm capacitation is associated with increased uptake of fluorescently labeled deoxyglucose and with extracellular acidification suggesting enhanced glycolysis. Consistently, in the present work we showed that glucose consumption is enhanced in media that support mouse sperm capacitation suggesting upregulation of glucose metabolic pathways. The increase in glucose consumption was modulated by bicarbonate and blocked by protein kinase A and soluble adenylyl cyclase inhibitors. Moreover, permeable cyclic adenosine monophosphate (cAMP) agonists increase glucose consumption in sperm incubated in conditions that do not support capacitation. Also, the increase in glucose consumption was reduced when sperm were incubated in low calcium conditions. Interestingly, this reduction was not overcome with cAMP agonists. Despite these findings, glucose consumption of sperm from Catsper1 knockout mice was similar to the one from wild type suggesting that other sources of calcium are also relevant. Altogether, these results suggest that cAMP and calcium pathways are involved in the regulation of glycolytic energy pathways during murine sperm capacitation.
Identifiants
pubmed: 32914502
doi: 10.1002/mrd.23421
pmc: PMC7730450
mid: NIHMS1645951
doi:
Substances chimiques
Calcium Channels
0
Catsper1 protein, mouse
0
Glucose
IY9XDZ35W2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1037-1047Subventions
Organisme : NICHD NIH HHS
ID : R01 HD038082
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD088571
Pays : United States
Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
Biol Reprod. 2020 Oct 5;103(4):791-801
pubmed: 32614044
Proc Natl Acad Sci U S A. 2004 Nov 23;101(47):16501-6
pubmed: 15546993
J Cell Physiol. 2018 Dec;233(12):9685-9700
pubmed: 29953592
Nature. 1951 Oct 20;168(4277):697-8
pubmed: 14882325
Biol Reprod. 2010 Jan;82(1):136-45
pubmed: 19759366
Reprod Domest Anim. 2012 Aug;47 Suppl 4:164-9
pubmed: 22827366
J Gen Physiol. 2003 Jul;122(1):115-28
pubmed: 12835474
J Biol Chem. 2015 Aug 14;290(33):20613-26
pubmed: 26048989
Biol Reprod. 2018 Nov 1;99(5):1010-1021
pubmed: 29893793
Aust J Sci Res B. 1951 Nov;4(4):581-96
pubmed: 14895481
Dev Cell. 2005 Aug;9(2):249-59
pubmed: 16054031
Development. 1995 Apr;121(4):1129-37
pubmed: 7743926
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
FEBS Lett. 2000 Jun 23;475(3):251-6
pubmed: 10869566
Mol Reprod Dev. 2016 Oct;83(10):860-874
pubmed: 27256723
J Cell Physiol. 2015 Aug;230(8):1758-1769
pubmed: 25597298
Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18543-8
pubmed: 24128762
Reprod Domest Anim. 2006 Oct;41 Suppl 2:11-20
pubmed: 16984465
J Biol Chem. 1999 Jan 29;274(5):3235-42
pubmed: 9915865
Front Cell Dev Biol. 2019 Nov 05;7:262
pubmed: 31750304
J Vis Exp. 2016 Nov 21;(117):
pubmed: 27911401
Sci Rep. 2016 Sep 15;6:33589
pubmed: 27627854
Dev Biol. 2006 Aug 15;296(2):353-62
pubmed: 16842770
Biol Reprod. 1999 Apr;60(4):973-8
pubmed: 10084974
FEBS Lett. 2002 Apr 10;516(1-3):229-33
pubmed: 11959138
Mol Hum Reprod. 1999 Jun;5(6):498-506
pubmed: 10340995
Adv Anat Embryol Cell Biol. 2016;220:35-69
pubmed: 27194349
Assay Drug Dev Technol. 2006 Oct;4(5):525-33
pubmed: 17115923
Dev Biol. 1999 Oct 15;214(2):429-43
pubmed: 10525345
J Cell Sci. 2005 May 1;118(Pt 9):2013-22
pubmed: 15840651
Nature. 2001 Oct 11;413(6856):603-9
pubmed: 11595941
Mol Hum Reprod. 2014 Nov;20(11):1054-66
pubmed: 25180269
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2993-8
pubmed: 14976244
Nat Chem Biol. 2016 Oct;12(10):838-44
pubmed: 27547922
J Biol Chem. 2010 Mar 12;285(11):7977-85
pubmed: 20068039
Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13483-8
pubmed: 15340140
Biol Reprod. 2012 Sep 28;87(3):75
pubmed: 22837480
Biol Reprod. 2003 May;68(5):1590-6
pubmed: 12606347
Expert Rev Proteomics. 2012 Oct;9(5):533-48
pubmed: 23194270