Synthetic media for preservation of corneal tissues deemed for endothelial keratoplasty and endothelial cell culture.
cell culture
cornea
endothelial cells
endothelial keratoplasty
endothelium
eye bank
media
preservation
synthetic
Journal
Acta ophthalmologica
ISSN: 1755-3768
Titre abrégé: Acta Ophthalmol
Pays: England
ID NLM: 101468102
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
04
07
2020
received:
15
01
2020
accepted:
09
07
2020
pubmed:
12
9
2020
medline:
1
12
2021
entrez:
11
9
2020
Statut:
ppublish
Résumé
To compare the difference between various endothelial graft preparation methods and endothelial cell culture from tissues that are preserved in serum-based and synthetic medium. In a randomized masked study, the tissues (n = 64) were preserved in Cornea Max (serum-based) and Cornea Syn (synthetic) series for 36 days at their respective preservation conditions. Following organ culture, corneal tissues (n = 48) were used to prepareDescemet stripping automated endothelial keratoplasty (DSAEK), preloaded ultra-thin (UT) -DSAEK, prestripped Descemet membrane endothelial keratoplasty (DMEK), free-floating DMEK, and preloaded DMEK with endothelium inward and outward grafts. These tissues were preserved for another 4days at room temperature in dextran supplemented media following which they were subjected to trypan blue, alizarin red, live/dead and Zonula Occludens-1 (ZO-1) staining. A separate set of tissues (n = 16) from both the series was used for human corneal endothelial cell (HCEnC) culture. At confluence, the proliferation and cell doubling rate was calculated and the cultured cells were subjected to live/dead, ZO-1, 2A12 and Ki-67 staining. Mann-Whitney test was performed with p < 0.05 deemed statistically significant. After preparation and preservation of the tissues for endothelial keratoplasty, alizarin red showed standard endothelial morphology from both the groups. Endothelial cell loss, hexagonality and uncovered areas did not show statistically significant differences (p > 0.05) between both groups. For HCEnC, cell doubling rate was 4.7 days (p > 0.05). All the antibodies were expressed in both the groups. Hexagonality, polymorphism, cell area, viable/dead cells and Ki-67 positivity were not statistically significant (p > 0.05). Complete synthetic organ culture series is safe and advantageous for carrying out advanced endothelial keratoplasty graft preparation procedures and for HCEnC culture as it is free from animal or animal-derived products.
Substances chimiques
Organ Preservation Solutions
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
314-325Subventions
Organisme : Italian Ministry of Health
ID : CUP: E46C18000340001
Informations de copyright
© 2020 The Authors. Acta Ophthalmologica published by John Wiley & Sons Ltd on behalf of Acta Ophthalmologica Scandinavica Foundation.
Références
Albon J, Tullo AB, Aktar S & Boulton ME (2000): Apoptosis in the endothelium of human corneas for transplantation. Invest Ophthalmol Vis Sci 41: 2887-2893.
Ayoubi MG, Armitage WJ & Easty DL (1996): Corneal organ culture: effects of serum and a stabilised form of L-glutamine. Br J Ophthalmol 80: 740-744.
Bednarz J, Doubilei V, Wollnik PC & Engelmann K (2001): Effect of three different media on serum free culture of donor corneas and isolated human corneal endothelial cells. Br J Ophthalmol 85: 1416-1420.
Camposampiero D, Tiso R, Zanetti E, Ruzza A, Bruni A & Ponzin D (2003): Cornea preservation in culture with bovine serum or chicken ovalbumin. Cornea 22: 254-258.
Ding V, Chin A, Peh G, Mehta JS & Choo A (2014): Generation of novel monoclonal antibodies for the enrichment and characterization of human corneal endothelial cells (hCENC) necessary for the treatment of corneal endothelial blindness. MAbs 6: 1439-1452.
Dirisamer M, Ham L, Dapena I, van Dijk K & Melles GR (2012): Descemet membrane endothelial transfer: "free-floating" donor Descemet implantation as a potential alternative to "keratoplasty”. Cornea 31: 194-197.
Ehlers H, Ehlers N & Hjortdal JO (1999): Corneal transplantation with donor tissue kept in organ culture for 7 weeks. Acta Ophthalmol Scand 77: 277-278.
Frueh BE & Bohnke M (1995): Corneal grafting of donor tissue preserved for longer than 4 weeks in organ-culture medium. Cornea 14: 463-466.
Gain P, Jullienne R, He Z, Aldossary M, Acquart S, Cognasse F & Thuret G (2016): Global survey of corneal transplantation and eye banking. JAMA Ophthalmol 134: 167-173.
Greenrod E, Jones M, Kaye S & Larkin F (2014): Center and surgeon effect on outcomes of endothelial keratoplasty versus penetrating keratoplasty in the United Kingdom. Am J Ophthalmol 158: 957-966.
Hempel B, Bednarz J & Engelmann K (2001): Use of a serum-free medium for long-term storage of human corneas. Influence on endothelial cell density and corneal metabolism. Graefes Arch Clin Exp Ophthalmol 239: 801-805.
Joyce NC (2012): Proliferative capacity of corneal endothelial cells. Exp Eye Res 95: 16-23.
Kimoto M, Shima N, Yamaguchi M, Amano S & Yamagami S (2012): Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic acid 2-phosphate. Invest Ophthalmol Vis Sci 53: 7583-7589.
Møller-Pedersen T, Hartmann U, Ehlers N & Engelmann K (2001a): Evaluation of potential organ culture media for eye banking using a human corneal endothelial cell growth assay. Graefes Arch Clin Exp Ophthalmol 239: 778-782.
Møller-Pedersen T, Hartmann U, Møller HJ, Ehlers N & Engelmann K (2001b): Evaluation of potential organ culture media for eye banking using human donor corneas. Br J Ophthalmol 85: 1075-1079.
Okumura N, Kay EP, Nakahara M, Hamuro J, Kinoshita S & Koizumi N (2013): Inhibition of TGF-beta signaling enables human corneal endothelial cell expansion in vitro for use in regenerative medicine. PLoS One 8: e58000.
Parekh M, Salvalaio G, Ruzza A, Camposampiero D, Griffoni C, Zampini A, Ponzin D & Ferrari S (2013): Posterior lamellar graft preparation: a prospective review from an eye bank on current and future aspects. J Ophthalmol 2013: 769860.
Parekh M, Ferrari S, Salvalaio G & Ponzin D (2015): Synthetic versus serum-based medium for corneal preservation in organ culture: a comparative study between 2 different media. Eur J Ophthalmol 25: 96-100.
Parekh M, Ruzza A, Ferrari S, Busin M & Ponzin D (2016): Preloaded tissues for Descemet membrane endothelial keratoplasty. Am J Ophthalmol 166: 120-125.
Parekh M, Ahmad S, Ruzza A & Ferrari S (2017a): Human corneal endothelial cell cultivation from old donor corneas with forced attachment. Sci Rep 7: 142.
Parekh M, Ruzza A, Ferrari S, Ahmad S, Kaye S, Ponzin D & Romano V (2017b): Endothelium-in versus endothelium-out for Descemet membrane endothelial keratoplasty graft preparation and implantation. Acta Ophthalmol 95: 194-198.
Parekh M, Ruzza A, Steger B, Willoughby CE, Ferrari S, Ponzin D, Kaye SB & Romano V (2017c): Avoiding complications associated with preloaded ultrathin Descemet stripping automated endothelial keratoplasty. Cornea 36: e12-e13.
Parekh M, Elbadawy H, Salvalaio G et al. (2018): Recombinant human serum albumin for corneal preservation. Acta Ophthalmol 96: e79-e86.
Parekh M, Peh G, Mehta JS, Ahmad S, Ponzin D & Ferrari S (2019a): Effects of corneal preservation conditions on human corneal endothelial cell culture. Exp Eye Res 179: 93-101.
Parekh M, Peh G, Mehta JS, Ramos T, Ponzin D, Ahmad S & Ferrari S (2019b): Passaging capability of human corneal endothelial cells derived from old donors with and without accelerating cell attachment. Exp Eye Res 189: 107814.
Parekh M, Romano V, Ruzza A, Kaye SB, Ponzin D, Ahmad S & Ferrari S (2019c): Culturing discarded peripheral human corneal endothelial cells from the tissues deemed for preloaded DMEK transplants. Cornea 38: 1175-1181.
Parekh M, Ruzza A, Ponzin D, Ahmad S & Ferrari S (2019d): Human corneal endothelial cell assessment from tissues preserved in serum-based and synthetic storage media. Cornea 38: 1438-1442.
Parekh M, Ruzza A, Steger B et al. (2019e): Cross-country transportation efficacy and clinical outcomes of preloaded large-diameter ultra-thin Descemet stripping automated endothelial keratoplasty grafts. Cornea 38: 30-34.
Peh GS, Beuerman RW, Colman A, Tan DT & Mehta JS (2011a): Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview. Transplantation 91: 811-819.
Peh GS, Toh KP, Wu FY, Tan DT & Mehta JS (2011b): Cultivation of human corneal endothelial cells isolated from paired donor corneas. PLoS One 6: e28310.
Peh GS, Chng Z, Ang HP et al. (2013a): Propagation of human corneal endothelial cells: a novel dual media approach. Cell Transplant 24: 287-304.
Peh GS, Toh KP, Ang HP, Seah XY, George BL & Mehta JS (2013b): Optimization of human corneal endothelial cell culture: density dependency of successful cultures in vitro. BMC Res Notes 6: 176.
Peh GS, Adnan K, George BL, Ang HP, Seah XY, Tan DT & Mehta JS (2015a): The effects of Rho-associated kinase inhibitor Y-27632 on primary human corneal endothelial cells propagated using a dual media approach. Sci Rep 5: 9167.
Peh GS, Ang HP, Lwin CN et al. (2015b): Regulatory compliant tissue-engineered human corneal endothelial grafts restore corneal function of rabbits with bullous keratopathy. Sci Rep 7: 14149.
Pels E & Schuchard Y (1983): Organ-culture preservation of human corneas. Doc Ophthalmol 56: 147-153.
Pels E & Schuchard Y(1984): The effects of high molecular weight dextran on the preservation of human corneas. Cornea 3: 219-227.
Pels E, Beele H & Claerhout I (2008): Eye bank issues: II. Preservation techniques: warm versus cold storage. Int Ophthalmol 28: 155-163.
Pipparelli A, Thuret G, Toubeau D, He Z, Piselli S, Lefevre S, Gain P & Muraine M (2011): Pan-corneal endothelial viability assessment: application to endothelial grafts predissected by eye banks. Invest Ophthalmol Vis Sci 52: 6018-6025.
Rieck PW, Gigon M, Jaroszewski J, Pleyer U & Hartmann C (2003): Increased endothelial survival of organ-cultured corneas stored in FGF-2-supplemented serumfree medium. Invest Ophthalmol Vis Sci 44: 3826-3832.
Romano V, Parekh M, Ruzza A, Willoughby CE, Ferrari S, Ponzin D, Kaye SB & Levis HJ (2018): Comparison of preservation and transportation protocols for preloaded Descemet membrane endothelial keratoplasty. Br J Ophthalmo 102: 549-555.
Ruzza A, Parekh M, Ferrari S, Salvalaio G, Nahum Y, Bovone C, Ponzin D & Busin M (2015): Preloaded donor corneal lenticules in a new validated 3D printed smart storage glide for Descemet stripping automated endothelial keratoplasty. Br J Ophthalmol 99: 1388-95.
Stoiber J, Ruckhofer J, Lametschwandtner A, Muss W, Hitzl W, Weikinger K & Grabner G (2001): Eurosol versus fetal bovine serumcontaining corneal storage medium. Cornea 20: 205-209.
Thuret G, Manissolle C, Campos-Guyotat L, Guyotat D & Gain P (2005): Animal compound-free medium and poloxamer for human corneal organ culture and deswelling. Invest Ophthalmol Vis Sci 46: 816-822.
Tourtas T, Laaser K, Bachmann B, Cursiefen C & Kruse F (2012): Descemet membrane endothelial keratoplasty versus Descemet stripping automated endothelial keratoplasty. Am J Ophthalmol 153: 1082-1090.
van der Valk J, Mellor D, Brands R et al. (2004): The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18: 1-12.
van der Valk J, Brunner D, De Smet K et al. (2010): Optimization of chemically defined cell culture media-replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro 24: 1053-1063.
van der Valk J, Bieback K, Buta C et al. (2018): Fetal Bovine Serum (FBS): Past - Present- Future. Altex 35: 99-118.