Selective Lysosome Membrane Turnover Is Induced by Nutrient Starvation.
Autophagy
Autophagy-Related Protein 5
/ metabolism
Cell Line
Endosomes
/ metabolism
Glucose
/ deficiency
Glutamine
/ metabolism
Humans
Intracellular Membranes
/ metabolism
Lipids
/ chemistry
Lysosomes
/ metabolism
Membrane Proteins
/ metabolism
Microtubule-Associated Proteins
/ metabolism
Nutrients
/ deficiency
ATG5
LAP
LC3
ammonium
autophagy
glucose
glutamine
lysosome
metabolism
microautophagy
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
09 11 2020
09 11 2020
Historique:
received:
18
03
2020
revised:
02
07
2020
accepted:
17
08
2020
pubmed:
12
9
2020
medline:
12
3
2021
entrez:
11
9
2020
Statut:
ppublish
Résumé
Lysosome function is essential for cellular homeostasis, but quality-control mechanisms that maintain healthy lysosomes remain poorly characterized. Here, we developed a method to measure lysosome turnover and use this to identify a selective mechanism of membrane degradation that involves lipidation of the autophagy protein LC3 onto lysosomal membranes and the formation of intraluminal vesicles through microautophagy. This mechanism is induced in response to metabolic stress resulting from glucose starvation or by treatment with pharmacological agents that induce osmotic stress on lysosomes. Cells lacking ATG5, an essential component of the LC3 lipidation machinery, show reduced ability to regulate lysosome size and degradative capacity in response to activation of this mechanism. These findings identify a selective mechanism of lysosome membrane turnover that is induced by stress and uncover a function for LC3 lipidation in regulating lysosome size and activity through microautophagy.
Identifiants
pubmed: 32916093
pii: S1534-5807(20)30666-3
doi: 10.1016/j.devcel.2020.08.008
pmc: PMC8337093
mid: NIHMS1723908
pii:
doi:
Substances chimiques
Autophagy-Related Protein 5
0
Lipids
0
MAP1LC3A protein, human
0
Membrane Proteins
0
Microtubule-Associated Proteins
0
Glutamine
0RH81L854J
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
289-297.e4Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA154649
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM111350
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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