Overexpression of Prolidase Induces Autophagic Death in MCF-7 Breast Cancer Cells.
Apoptosis
/ drug effects
Autophagic Cell Death
/ drug effects
Autophagy
/ drug effects
Breast Neoplasms
/ metabolism
Cell Survival
/ drug effects
Cells, Cultured
Collagen
/ metabolism
Dipeptidases
/ metabolism
Fibroblasts
/ metabolism
Humans
MCF-7 Cells
/ metabolism
Proline
/ pharmacology
Proline Oxidase
/ metabolism
Prolidase; Proline; Autophagy; Collagen biosynthesis; Breast cancer cells MCF-7
Journal
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
ISSN: 1421-9778
Titre abrégé: Cell Physiol Biochem
Pays: Germany
ID NLM: 9113221
Informations de publication
Date de publication:
12 Sep 2020
12 Sep 2020
Historique:
accepted:
02
09
2020
entrez:
12
9
2020
pubmed:
13
9
2020
medline:
26
1
2021
Statut:
ppublish
Résumé
Proline availability for proline dehydrogenase/proline oxidase (PRODH/POX) may represent switching mechanism between PRODH/POX-dependent apoptosis and autophagy. The aim of the study was to evaluate the impact of overexpression of prolidase (proline releasing enzyme) on apoptosis/autophagy in breast cancer MCF-7 cells. The model of MCF-7 cells with prolidase overexpression (MCF-7 Prolidase overexpression in MCF-7 The data suggest that overexpression of prolidase in MCF-7 cells contributes to increase in intracellular proline concentration and PRODH/POX-dependent autophagic cell death.
Sections du résumé
BACKGROUND/AIMS
OBJECTIVE
Proline availability for proline dehydrogenase/proline oxidase (PRODH/POX) may represent switching mechanism between PRODH/POX-dependent apoptosis and autophagy. The aim of the study was to evaluate the impact of overexpression of prolidase (proline releasing enzyme) on apoptosis/autophagy in breast cancer MCF-7 cells.
METHODS
METHODS
The model of MCF-7 cells with prolidase overexpression (MCF-7
RESULTS
RESULTS
Prolidase overexpression in MCF-7
CONCLUSION
CONCLUSIONS
The data suggest that overexpression of prolidase in MCF-7 cells contributes to increase in intracellular proline concentration and PRODH/POX-dependent autophagic cell death.
Substances chimiques
Collagen
9007-34-5
Proline
9DLQ4CIU6V
Proline Oxidase
EC 1.5.3.-
Dipeptidases
EC 3.4.13.-
proline dipeptidase
EC 3.4.13.9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
875-887Subventions
Organisme : National Science Center (NCN)
ID : 2017/25/B/NZ7/02183
Pays : Poland
Organisme : The European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant and the Polish Ministry of Science and Higher Education, from financial resources for science in 2018-2023 granted for the implementation of an international co-financed project
Pays : Poland
Informations de copyright
© Copyright by the Author(s). Published by Cell Physiol Biochem Press.
Déclaration de conflit d'intérêts
The authors have no conflicts of interest to declare.