Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar.
EV-A71
HFMD
enterovirus 71
molecular detection
new genogroups
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
20
05
2020
accepted:
21
07
2020
entrez:
14
9
2020
pubmed:
15
9
2020
medline:
15
9
2020
Statut:
epublish
Résumé
Enterovirus A71 (EV-A71) is a leading cause of hand-foot-and-mouth disease (HFMD) and can be associated with severe neurological complications. EV-A71 strains can be classified into seven genogroups, A-H, on the basis of the VP1 capsid protein gene sequence. Genogroup A includes the prototype strain; genogroups B and C are responsible of major outbreaks worldwide, but little is known about the others, particularly genogroups E and F, which have been recently identified in Africa and Madagascar, respectively. The circulation of EV-A71 in the African region is poorly known and probably underestimated. A rapid and specific assay for detecting all genogroups of EV-A71 is required. In this study, we developed a real-time RT-PCR assay with a competitive internal control (IC). The primers and
Identifiants
pubmed: 32922374
doi: 10.3389/fmicb.2020.01907
pmc: PMC7456875
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1907Informations de copyright
Copyright © 2020 Volle, Joffret, Ndiaye, Fernandez-Garcia, Razafindratsimandresy, Heraud, Rezig, Sadeuh-Mba, Boulahbal-Anes, Seghier, Deshpandeh, Bessaud and Delpeyroux.
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