High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Aug 2020
01 Aug 2020
Historique:
received:
30
03
2020
revised:
01
06
2020
accepted:
02
06
2020
entrez:
14
9
2020
pubmed:
15
9
2020
medline:
15
9
2020
Statut:
epublish
Résumé
Single-cell analysis techniques are fundamental to study the heterogeneity of cellular populations, which is the basis to understand several biomedical mechanisms. Light-sheet fluorescence microscopy is a powerful technique for obtaining high-resolution imaging of individual cells, but the complexity of the setup and the sample mounting procedures limit its overall throughput. In our work, we present an optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size. The device is used to perform dual-color fluorescence analysis and 3D reconstruction of xenograft-derived mouse breast cancer cells.
Identifiants
pubmed: 32923051
doi: 10.1364/BOE.393892
pii: 393892
pmc: PMC7449749
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4397-4407Informations de copyright
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest related to this article.
Références
Lab Chip. 2016 Apr 26;16(9):1556-60
pubmed: 27030116
Cell Tissue Res. 2013 Apr;352(1):161-77
pubmed: 23443300
Biomed Opt Express. 2016 May 17;7(6):2219-36
pubmed: 27375939
Cytometry A. 2017 Feb;91(2):144-151
pubmed: 28075531
Cytometry A. 2018 Oct;93(10):987-996
pubmed: 30211977
Lab Chip. 2014 Jun 7;14(11):1826-33
pubmed: 24740611
Biomed Opt Express. 2018 Jun 27;9(7):3424-3433
pubmed: 29984107
Nat Methods. 2010 Aug;7(8):637-42
pubmed: 20601950
Nat Methods. 2011 Jul 17;8(9):757-60
pubmed: 21765409
Nat Protoc. 2015 Aug;10(8):1234-47
pubmed: 26203821
Opt Express. 2013 Jun 17;21(12):14474-80
pubmed: 23787635
Opt Express. 2017 Apr 3;25(7):7313-7323
pubmed: 28380855
IEEE Trans Image Process. 2007 Dec;16(12):2992-3004
pubmed: 18092598
Biomed Opt Express. 2015 Oct 19;6(11):4447-56
pubmed: 26601007
Opt Express. 2009 May 11;17(10):8685-95
pubmed: 19434202
Nat Methods. 2015 Jul;12(7):641-4
pubmed: 25961414
Science. 2004 Aug 13;305(5686):1007-9
pubmed: 15310904
Nat Commun. 2018 Mar 9;9(1):1024
pubmed: 29523784