MCM5 urine expression (ADXBLADDER) is a reliable biomarker of high-risk non- muscle-invasive bladder cancer recurrence: A prospective matched case-control study.


Journal

Cancer biomarkers : section A of Disease markers
ISSN: 1875-8592
Titre abrégé: Cancer Biomark
Pays: Netherlands
ID NLM: 101256509

Informations de publication

Date de publication:
2021
Historique:
pubmed: 15 9 2020
medline: 21 10 2021
entrez: 14 9 2020
Statut: ppublish

Résumé

Mini Chromosome Maintenance 5 (MCM5) is considered as a urinary biomarker of bladder cancer. ADXBLADDER is a commercially available test to detect MCM5 antibodies. External validation of ADXBLADDER test as a urinary biomarker of histopathologically confirmed non-muscle invasive bladder cancer (NMIBC) recurrence. The study enrolled 119 consecutive patients with a history of NMIBC and 37 healthy volunteers matched as controls. Single, full-void urine samples were collected from patients before cystoscopy ± TUR. To measure MCM5 expression, Arquer Diagnostics ADXBLADDER test was used. The study protocol was registered within the clinical trials database (NCT03796299). Among patients with NMIBC history, recurrence was diagnosed in 83 patients (69.7%). ADXBLADDER demonstrated sensitivity of 73.5% (95% confidence interval (CI) 62.7%-82.6%), specificity of 33.3% (95% CI 18.6% to 51%), overall negative predictive value (NPV) of 35.3% (95% CI 23.3% to 49.5%) and overall positive predictive value of 71.8% (95% CI 66.1% to 76.8%) for detecting recurrence. In a control group, false positive ADXBLADDER results were noticed in 18 patients (48.6%). The sensitivity and NPV were the highest in invasive tumors (100% and 100%, respectively) and in high-grade recurrences (81.8% and 94.1%, respectively). ADXBLADDER has a moderate sensitivity and poor specificity in detecting NMIBC recurrence. However, it properly diagnoses patients with T1+ stage recurrence or high-grade tumors.

Sections du résumé

BACKGROUND BACKGROUND
Mini Chromosome Maintenance 5 (MCM5) is considered as a urinary biomarker of bladder cancer. ADXBLADDER is a commercially available test to detect MCM5 antibodies.
OBJECTIVE OBJECTIVE
External validation of ADXBLADDER test as a urinary biomarker of histopathologically confirmed non-muscle invasive bladder cancer (NMIBC) recurrence.
METHODS METHODS
The study enrolled 119 consecutive patients with a history of NMIBC and 37 healthy volunteers matched as controls. Single, full-void urine samples were collected from patients before cystoscopy ± TUR. To measure MCM5 expression, Arquer Diagnostics ADXBLADDER test was used. The study protocol was registered within the clinical trials database (NCT03796299).
RESULTS RESULTS
Among patients with NMIBC history, recurrence was diagnosed in 83 patients (69.7%). ADXBLADDER demonstrated sensitivity of 73.5% (95% confidence interval (CI) 62.7%-82.6%), specificity of 33.3% (95% CI 18.6% to 51%), overall negative predictive value (NPV) of 35.3% (95% CI 23.3% to 49.5%) and overall positive predictive value of 71.8% (95% CI 66.1% to 76.8%) for detecting recurrence. In a control group, false positive ADXBLADDER results were noticed in 18 patients (48.6%). The sensitivity and NPV were the highest in invasive tumors (100% and 100%, respectively) and in high-grade recurrences (81.8% and 94.1%, respectively).
CONCLUSIONS CONCLUSIONS
ADXBLADDER has a moderate sensitivity and poor specificity in detecting NMIBC recurrence. However, it properly diagnoses patients with T1+ stage recurrence or high-grade tumors.

Identifiants

pubmed: 32924986
pii: CBM200316
doi: 10.3233/CBM-200316
doi:

Substances chimiques

Biomarkers, Tumor 0
Cell Cycle Proteins 0
MCM5 protein, human 0

Banques de données

ClinicalTrials.gov
['NCT03796299']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

139-143

Auteurs

Łukasz Białek (Ł)

First Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.

Katarzyna Czerwińska (K)

Department of Transplantation Medicine, Nephrology and Internal Diseases, Medical University of Warsaw, Warsaw, Poland.

Łukasz Fus (Ł)

Department of Pathology, Medical University of Warsaw, Warsaw, Poland.

Wojciech Krajewski (W)

Department and Clinic of Urology, Medical University of Wroclaw, Wroclaw, Poland.

Anna Sadowska (A)

Department of Transplantation Medicine, Nephrology and Internal Diseases, Medical University of Warsaw, Warsaw, Poland.

Piotr Radziszewski (P)

Department of General, Oncological and Functional Urology, Medical University of Warsaw, Warsaw, Poland.

Jakub Dobruch (J)

First Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.

Piotr Kryst (P)

Second Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.

Sławomir Poletajew (S)

Second Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.

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Classifications MeSH