High-Dimensional Flow Cytometry Analysis of Regulatory Receptors on Human T Cells, NK Cells, and NKT Cells.
Adaptive immune cells
Immune checkpoint
Innate immune cells
Memory markers
NK cell
NK receptor
NKT cell
T cell
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
14
9
2020
pubmed:
15
9
2020
medline:
1
4
2021
Statut:
ppublish
Résumé
The field of flow cytometry has witnessed rapid technological advancements in the last few decades. While the founding principles of fluorescent detection on cells (or particles) within a uniform fluid stream remains largely unchanged, the availability more sensitive cytometers with the ability to multiplex more and more florescent signals has resulted in very complex high-order assays. This results in the co-use of fluorophores with increased levels of emission overlap and/or spillover spreading than in years past and thus requires careful and well thought out planning for flow cytometry assay development. As an example, we present the development of a large 18-color (20 parameter) flow cytometry assay designed to take an in depth analysis of effector lymphocyte phenotypes, with careful attention to assay controls and panel design.
Identifiants
pubmed: 32926371
doi: 10.1007/978-1-0716-0849-4_14
pmc: PMC7744271
mid: NIHMS1650769
doi:
Substances chimiques
Antibodies
0
Immune Checkpoint Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
255-290Subventions
Organisme : NCI NIH HHS
ID : P30 CA076292
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA168536
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA148995
Pays : United States
Références
Nat Immunol. 2010 Mar;11(3):197-206
pubmed: 20139988
Nat Rev Immunol. 2013 Feb;13(2):145-9
pubmed: 23348417
J Clin Invest. 2007 Nov;117(11):3383-92
pubmed: 17932562
Oncotarget. 2016 Nov 8;7(45):72961-72977
pubmed: 27662664
Science. 1999 Jul 30;285(5428):727-9
pubmed: 10426993
Cancer Res. 1997 Sep 15;57(18):4036-41
pubmed: 9307290
Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10984-9
pubmed: 20534460
J Infect Dis. 2010 Aug 15;202(4):524-32
pubmed: 20617899
J Immunol. 2014 Apr 15;192(8):3805-15
pubmed: 24646734
Nature. 2015 Jan 15;517(7534):293-301
pubmed: 25592534
Blood. 2014 Jul 31;124(5):700-9
pubmed: 24778151
Cancer Res. 2011 May 15;71(10):3540-51
pubmed: 21430066
Blood. 2012 Apr 19;119(16):3734-43
pubmed: 22383801
Adv Immunol. 2014;122:91-128
pubmed: 24507156
Eur J Immunol. 2002 Mar;32(3):634-43
pubmed: 11857337
Eur J Immunol. 2005 May;35(5):1399-407
pubmed: 15816002
Nat Immunol. 2011 Jan;12(1):21-7
pubmed: 21113163
J Allergy Clin Immunol. 2016 Nov;138(5):1253-1264
pubmed: 27817797
Nat Rev Immunol. 2006 Jul;6(7):520-31
pubmed: 16799471
J Immunother. 2012 Nov-Dec;35(9):651-60
pubmed: 23090074
Blood. 2012 Mar 29;119(13):3064-72
pubmed: 22323453
Nat Rev Immunol. 2014 Jun;14(6):377-91
pubmed: 24830344
Front Immunol. 2017 Mar 16;8:293
pubmed: 28360915
J Leukoc Biol. 1999 Sep;66(3):375-81
pubmed: 10496306
Inflamm Bowel Dis. 2013 May;19(6):1330-8
pubmed: 23518808
Immunol Lett. 2016 Nov;179:2-8
pubmed: 26844414
Eur J Immunol. 2011 Jul;41(7):1913-23
pubmed: 21590763
Nat Immunol. 2005 Dec;6(12):1245-52
pubmed: 16286920
Nat Immunol. 2018 Jul;19(7):723-732
pubmed: 29915296
Trends Immunol. 2001 Nov;22(11):633-40
pubmed: 11698225
J Exp Med. 1997 Jul 7;186(1):109-20
pubmed: 9207002
Science. 1995 May 12;268(5212):863-5
pubmed: 7538697
J Exp Med. 1999 Jun 21;189(12):1973-80
pubmed: 10377193
Eur J Immunol. 2001 Apr;31(4):1076-86
pubmed: 11298332
J Allergy Clin Immunol. 2017 Jan;139(1):335-346.e3
pubmed: 27372564
Nat Rev Immunol. 2018 Jun;18(6):363-373
pubmed: 29520044
J Clin Invest. 2011 Apr;121(4):1456-70
pubmed: 21364281
J Immunol. 2013 Mar 1;190(5):2424-36
pubmed: 23359508
Nat Immunol. 2001 Mar;2(3):261-8
pubmed: 11224527
J Exp Med. 1995 Dec 1;182(6):2091-6
pubmed: 7500054
Immunity. 2018 Mar 20;48(3):417-433
pubmed: 29562193