A study to compare Hematopoietic Progenitor Cell count determined on a next-generation automated cell counter with flow cytometric CD34 count in peripheral blood and the harvested peripheral blood stem cell graft from autologous and allogenic donors.
CD34
hematopoietic progenitor cells
stem cell graft
Journal
International journal of laboratory hematology
ISSN: 1751-553X
Titre abrégé: Int J Lab Hematol
Pays: England
ID NLM: 101300213
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
21
05
2020
revised:
17
08
2020
accepted:
23
08
2020
pubmed:
15
9
2020
medline:
7
5
2021
entrez:
14
9
2020
Statut:
ppublish
Résumé
A successful bone marrow transplant requires a minimum of 2-4 × 10 Autologous and allogenic donors were taken as per criteria. TLC (Total Leukocyte Count), MNC (Mononuclear cell count), HPC, and CD34 assay were done in both the peripheral blood prior to apheresis, and the harvest product postapheresis. Sysmex XN-9000 was used for TLC, MNC, and HPC tests, and a modified ISH-AGE protocol was used to enumerate CD34 by flow cytometry. Statistical analysis was done using SPSS 16.0. Sixty-seven allogenic and 35 autologous donors were enrolled. 45% were females, and 55% were males. Correlation between HPC and CD34 was found to be 0.887 with P value < .01 in peripheral blood and 0.847 with P value < .01 in the harvested product. On the other hand, TLC had a correlation of 0.424 and 0.520 in peripheral blood and harvested, respectively. MNC had a weak association. The cutoff value for a target dose of 2 × 10 We conclude that HPC is comparable to CD34 in predicting harvest product's adequacy.
Types de publication
Clinical Trial
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
76-83Informations de copyright
© 2020 John Wiley & Sons Ltd.
Références
Gratwohl A, Baldomero H, Passweg J, et al. Hematopoietic stem cell transplantation for hematological malignancies in Europe. Leukemia. 2003;17:941-959.
Korbling M, Freireich EJ. Twenty-five years of peripheral blood stem cell transplantation. Blood. 2011;117:6411-6416.
Koca E, Champlin RE. Peripheral blood progenitor cell or bone marrow transplantation: controversy remains. Curr Opin Oncol. 2008;20:220-226.
Schmitz N, Barrett J. Optimizing engraftment-source and dose of stem cells. Semin Hematol. 2002;39:3-14.
Bender JG, To LB, Williams S, et al. Defining a therapeutic dose of peripheral blood stem cells. J Hematother. 1992;1:329-334.
Zimmerman TM, Lee WJ, Bender JG, et al. Quantitative CD34 analysis may be used to guide peripheral blood stem cell harvests. Bone Marrow Transplant. 1995;15:439-444.
Armitage S, Hargreaves R, Samson D, et al. CD 34 counts to predict the adequate collection of peripheral blood progenitor cells. Bone Marrow Transplant. 1997;20:587-591.
Peerschke EI, Moung C, Pessin MP, Maslak P. Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections. Transfusion. 2015;55(8):2001-2009.
Tanosaki R, et al. Novel and rapid enumeration method of peripheral blood stem cells using automated hematology analyzer. Int. Jnl. Lab. Hem. 2014;36:521-530.
Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematother. 1996;5:213-226.
Barnett D, Janossy G, Lubenko A, Matutes E, Newland A, Reilly JT. Guideline for the flow cytometric enumeration of CD34+ haematopoietic stem cells. Prepared by the CD34+ haematopoietic stem cell working party. General Haematology Task Force of the British Committee for Standards in Haematology. Clin Lab Haematol. 1999;21(5):301-308.
Yu J, Leisenring W, Rowley SD, et al. The predictive value of white cell or CD34+ cell count in peripheral blood for timing of apheresis and maximizing yield. Transfusion. 1999;39:442-450.
Elliott C, Samson DM, Armitage S, et al. When to harvest peripheral blood stem cells after mobilization therapy: prediction of CD34-positive cell yield by preceding day CD34-positive concentration in peripheral blood. J Clin Oncol. 1996;14:970-973.
Schots R, Van Riet I, Damiaens S, et al. The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis. Bone Marrow Transplant. 1996;17:509-515.
Shalel S, Streichman S, Marmur A. The mechanism of hemolysis by surfactants: effect of solution composition. J Colloid Interface Sci. 2002;252:66-76.
Sanchez L, Martinez V, Infante MR, Mitjans M, Vinardell MP. Hemolysis and antihemolysis induced by amino acid-based surfactants. Toxicol Lett. 2007;169:177-184.
Vogel W, Kopp HG, Kanz L, et al. Correlations between hematopoietic progenitor cell counts as measured by Sysmex and CD34+ cell harvest yields following mobilization with different regimens. J Cancer Res Clin Oncol. 2002;128:380-384.
Yang SH, Wang TF, Tsai HH, et al. Preharvest hematopoietic progenitor cell counts predict CD34+ cell yields in granulocyte-colony-stimulating factor mobilized peripheral blood stem cell harvest in healthy donors. Transfusion. 2010;50:1088-1095.
Kraai R, Reymer AG, Brouwer-Mandema GG, et al. Hemopoietic stem and precursor cell analysis in umbilical cord blood using the Sysmex SE-9000 IMI channel. Cytometry. 2001;46:114-118.
Letestu R, Marzac C, Audat F, et al. Use of hematopoietic progenitor cell count on the Sysmex XE- 2100 for peripheral blood stem cell harvest monitoring. Leuk Lymphoma. 2007;48:89-96.
Mougi H, Shinmyozu K, Osame M. Determining the optimal time for peripheral blood stem cell harvest by detecting immature cells (immature leukocytes and immature reticulocytes) using two newly developed automatic cell analyzers. Int J Hematol. 1997;66:303-313.
Oelschlaegel U, Bornhaeuser M, Thiede C, Ehninger G, Hoelig K. HPC enumeration with the Sysmex XE-2100 can guide further flow cytometric CD34(+) measurements and timing of leukapheresis. Cytotherapy. 2003;5:414-419.
Park SH, et al. The New Sysmex XN-2000 automated blood cell analyzer more accurately measures the absolute number and the proportion of hematopoietic stem and progenitor cells than XE-2100 when compared to flow cytometric enumeration of CD34+ cells. Ann Lab Med. 2015;35:146-148.
Gromme M, Russcher H, Braakman E, et al. Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management. Transfusion. 2017;57:1949-1955.
Tanosaki R, Okuyama Y, Iseki T, et al. Enumeration of peripheral blood hematopoietic stem cells using automated haematology analyzer, Sysmex-XN: final results of multicentre prospective study. Blood. 2015;126:4304.
Kumar K, Subash CS. A study to identify correlation of hematopoietic progenitor cell (XN-HPC) By sysmex cell counters with final yield of CD34 + stem cells. Blood. 2017;130:5458.
Furundarena JR, Uranga A, Alkorta A, et al. Evaluation of the predictive value of the hematopoietic progenitor cell count using an automated hematology analyzer for CD34+ stem cell mobilization and apheresis product yield. Int J Lab Hematol. 2020;42(2):170-179. https://doi.org/10.1111/ijlh.13142