A Simple Drug Delivery System for Platelet-Derived Bioactive Molecules, to Improve Melanocyte Stimulation in Vitiligo Treatment.
centrifugal spinning
electrospinning
melanocyte
platelets
vitiligo
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
10 Sep 2020
10 Sep 2020
Historique:
received:
14
08
2020
revised:
02
09
2020
accepted:
08
09
2020
entrez:
15
9
2020
pubmed:
16
9
2020
medline:
16
9
2020
Statut:
epublish
Résumé
Vitiligo is the most common depigmentation disorder of the skin. Currently, its therapy focuses on the halting of the immune response and stimulation of the regenerative processes, leading to the restoration of normal melanocyte function. Platelet-rich plasma (PRP) represents a safe and cheap regenerative therapy option, as it delivers a wide spectrum of native growth factors, cytokines and other bioactive molecules. The aim of this study was to develop a simple delivery system to prolong the effects of the bioactive molecules released from platelets. The surface of electrospun and centrifugally spun poly-ε-caprolactone (PCL) fibrous scaffolds was functionalized with various concentrations of platelets; the influence of the morphology of the scaffolds and the concentration of the released platelet-derived bioactive molecules on melanocytes, was then assessed. An almost two-fold increase in the amount of the released bioactive molecules was detected on the centrifugally spun vs. electrospun scaffolds, and a sustained 14-day release of the bioactive molecules was demonstrated. A strong concentration-dependent response of melanocyte to the bioactive molecules was observed; higher concentrations of bioactive molecules resulted in improved metabolic activity and proliferation of melanocytes. This simple system improves melanocyte viability, offers on-site preparation and is suitable for prolonged topical PRP administration.
Identifiants
pubmed: 32927642
pii: nano10091801
doi: 10.3390/nano10091801
pmc: PMC7559479
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : LO1508
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : CZ.2.16/3.1.00/21528
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : CEITEC 2020 (LQ1601)
Organisme : CzechNanoLab Research Infrastructure
ID : LM2018110
Organisme : Agentura Pro Zdravotnický Výzkum České Republiky
ID : 17-32285A
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