Isolation and Characterization of Two Novel Colorectal Cancer Cell Lines, Containing a Subpopulation with Potential Stem-Like Properties: Treatment Options by MYC/NMYC Inhibition.
ATRA
EMT
KJ-Pyr-9
MYC
NMYC
colorectal adenocarcinoma
colorectal cancer stem cells
rectal neuroendocrine carcinoma
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
10 Sep 2020
10 Sep 2020
Historique:
received:
14
08
2020
revised:
04
09
2020
accepted:
08
09
2020
entrez:
15
9
2020
pubmed:
16
9
2020
medline:
16
9
2020
Statut:
epublish
Résumé
Cancer stem cells (CSC) are crucial mediators of cancer relapse. Here, we isolated two primary human colorectal cancer cell lines derived from a rectal neuroendocrine carcinoma (BKZ-2) and a colorectal adenocarcinoma (BKZ-3), both containing subpopulations with potential stem-like properties. Protein expression of CSC-markers prominin-1 and CD44 antigen was significantly higher for BKZ-2 and BKZ-3 in comparison to well-established colon carcinoma cell lines. High sphere-formation capacity further confirmed the existence of a subpopulation with potential stem-like phenotype. Epithelial-mesenchymal transition markers as well as immune checkpoint ligands were expressed more pronounced in BKZ-2. Both cell populations demonstrated N-myc proto-oncogene (
Identifiants
pubmed: 32927768
pii: cancers12092582
doi: 10.3390/cancers12092582
pmc: PMC7564713
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
Cell Cycle. 2018;17(12):1457-1470
pubmed: 29954240
Cell Death Dis. 2018 Aug 30;9(9):896
pubmed: 30166520
Development. 2016 Oct 15;143(20):3639-3649
pubmed: 27802133
Cancer Sci. 2019 Dec;110(12):3708-3717
pubmed: 31648389
Digestion. 2000;62(4):262-70
pubmed: 11070410
Ann Coloproctol. 2018 Aug;34(4):222-225
pubmed: 30048995
Cancers (Basel). 2019 May 11;11(5):
pubmed: 31083587
J Cell Physiol. 2019 Jun;234(6):8192-8202
pubmed: 30317669
Oncol Rep. 2013 Oct;30(4):1645-50
pubmed: 23877261
Oncol Lett. 2019 May;17(5):4487-4493
pubmed: 30944638
Sci Rep. 2019 Jul 1;9(1):9457
pubmed: 31263239
Cancers (Basel). 2019 Jul 17;11(7):
pubmed: 31319569
Nat Rev Neurosci. 2011 Apr;12(4):204-16
pubmed: 21415847
ISRN Oncol. 2012;2012:139268
pubmed: 23209942
Physiol Res. 2015;64(4):561-9
pubmed: 25470521
Cancer Cell. 2016 Oct 10;30(4):563-577
pubmed: 27728805
Br J Cancer. 2013 Apr 30;108(8):1720-31
pubmed: 23538387
Mod Pathol. 2002 Feb;15(2):159-66
pubmed: 11850545
J Nutr Biochem. 2013 May;24(5):744-53
pubmed: 22854319
Oncotarget. 2017 Dec 22;9(70):33403-33415
pubmed: 30279970
Eur J Endocrinol. 2010 Feb;162(2):391-8
pubmed: 20097833
Life Sci. 2017 Sep 1;184:25-29
pubmed: 28697984
Gastroenterology. 2010 Jun;138(6):2151-62
pubmed: 20420952
Nat Rev Mol Cell Biol. 2006 Feb;7(2):131-42
pubmed: 16493418
Clin Colorectal Cancer. 2018 Sep;17(3):e579-e592
pubmed: 29921496
Asian J Surg. 2018 May;41(3):274-278
pubmed: 28190751
Medicine (Baltimore). 2019 Dec;98(49):e18174
pubmed: 31804333
Brain Res Mol Brain Res. 1994 Dec;27(2):281-9
pubmed: 7898312
Dis Colon Rectum. 1994 Jul;37(7):635-42
pubmed: 8026228
PLoS One. 2014 Feb 14;9(2):e88713
pubmed: 24551139
Cell Stem Cell. 2020 Apr 2;26(4):569-578.e7
pubmed: 32169167
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Neuroendocrinology. 2018;107(3):246-256
pubmed: 30025411
Oncotarget. 2017 Oct 10;8(58):98635-98645
pubmed: 29228716
Cancer Lett. 2012 Jun 28;319(2):182-189
pubmed: 22261335
Oncoimmunology. 2017 Jan 23;6(1):e1263412
pubmed: 28197390
Nat Genet. 2013 Oct;45(10):1127-33
pubmed: 24071851
J Control Release. 2018 Jan 10;269:405-422
pubmed: 29170140
Int J Mol Sci. 2019 May 11;20(9):
pubmed: 31083525
Cell Rep. 2019 Jul 30;28(5):1282-1295.e8
pubmed: 31365870
Science. 2016 Apr 8;352(6282):227-31
pubmed: 26966191
Am J Pathol. 2007 Aug;171(2):386-95
pubmed: 17600130
Exp Hematol Oncol. 2013 Jun 06;2(1):16
pubmed: 23738757
Nature. 2002 Jun 27;417(6892):949-54
pubmed: 12068308
Cells. 2019 Aug 18;8(8):
pubmed: 31426611
Eur J Cancer. 2012 Nov;48(17):3310-8
pubmed: 22640830
Semin Cancer Biol. 2015 Apr;31:28-35
pubmed: 25025713
Proc Natl Acad Sci U S A. 1986 May;83(10):3500-4
pubmed: 3010302
Biochim Biophys Acta Rev Cancer. 2018 Dec;1870(2):229-238
pubmed: 29981816
Stem Cells Dev. 2012 Jul 1;21(10):1822-30
pubmed: 21973238
Int J Biochem Cell Biol. 2019 Jan;106:1-7
pubmed: 30399449
Tohoku J Exp Med. 2013;229(4):301-9
pubmed: 23615455
Front Surg. 2018 Jan 22;4:76
pubmed: 29404335
Mol Cell Biol. 2013 Aug;33(15):2930-49
pubmed: 23716601
Mod Pathol. 2019 Jul;32(7):929-942
pubmed: 30760860
Oncotarget. 2018 Nov 23;9(92):36503-36514
pubmed: 30559933
Oncol Rep. 2019 Jul;42(1):386-398
pubmed: 31059103
Am J Cancer Res. 2019 Feb 01;9(2):434-448
pubmed: 30906641
Cell. 2012 Mar 30;149(1):22-35
pubmed: 22464321
PLoS One. 2015 Oct 01;10(10):e0139727
pubmed: 26426996
Oncotarget. 2016 Nov 29;7(48):78499-78515
pubmed: 27655682
Crit Rev Oncol Hematol. 2014 Oct;92(1):11-24
pubmed: 24952997
J Clin Endocrinol Metab. 2016 Oct;101(10):3564-3567
pubmed: 27583474
Cancer Sci. 2019 Jan;110(1):379-388
pubmed: 30375704
Nat Cell Biol. 2009 Feb;11(2):162-71
pubmed: 19136966
Anticancer Res. 2009 Jun;29(6):1951-61
pubmed: 19528452
Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12556-61
pubmed: 25114221
Mol Cancer Res. 2017 Jun;15(6):744-752
pubmed: 28179411
Cell Signal. 2014 Oct;26(10):2234-9
pubmed: 25025570
World J Gastroenterol. 2018 Sep 7;24(33):3749-3759
pubmed: 30197480
Oncol Rep. 2016 Mar;35(3):1868-74
pubmed: 26717879
Cancers (Basel). 2019 Mar 27;11(4):
pubmed: 30934773
Discov Med. 2017 Apr;23(127):235-245
pubmed: 28595036
Nat Med. 2015 May;21(5):457-66
pubmed: 25849135
Cancer Sci. 2015 Jul;106(7):803-11
pubmed: 25940879
Cancer Cell. 2016 Apr 11;29(4):536-547
pubmed: 27050099
Biomed Pharmacother. 2014 Jun;68(5):507-13
pubmed: 24751002
Cell Cycle. 2005 Jun;4(6):772-6
pubmed: 15917668
Cell Physiol Biochem. 2018;46(2):860-872
pubmed: 29627827
Anticancer Res. 2010 Dec;30(12):4915-20
pubmed: 21187470
Cancer Sci. 2017 Mar;108(3):283-289
pubmed: 28064442
PLoS One. 2013 Jul 29;8(7):e70858
pubmed: 23923027
Cancer Sci. 2010 Jul;101(7):1731-7
pubmed: 20384634
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Oncogene. 2016 Oct 27;35(43):5619-5628
pubmed: 27157616
Oncotarget. 2018 Oct 5;9(78):34658-34669
pubmed: 30410666
J Clin Pathol. 2018 Feb;71(2):110-116
pubmed: 28942428
Cancer Res. 2009 Feb 1;69(3):1016-26
pubmed: 19155306
Oncotarget. 2017 Jun 13;8(24):39605-39617
pubmed: 28465486
PLoS One. 2012;7(8):e41335
pubmed: 22912670
Stem Cells Transl Med. 2015 Sep;4(9):1033-43
pubmed: 26136504
J Clin Invest. 2019 Jul 1;129(9):3924-3940
pubmed: 31260412
Onco Targets Ther. 2018 Nov 01;11:7635-7642
pubmed: 30464512
Clin Gastroenterol Hepatol. 2019 Feb;17(3):455-462.e6
pubmed: 29660527
J Dig Dis. 2017 Oct;18(10):574-581
pubmed: 28851046
Cancer Res. 2012 Oct 1;72(19):5101-10
pubmed: 23002207
Int J Oncol. 2016 Jan;48(1):349-57
pubmed: 26548461
PLoS One. 2012;7(9):e46665
pubmed: 23029563
Cancer Res. 2009 Apr 15;69(8):3382-9
pubmed: 19336570