Marker Assisted Development and Characterization of Herbicide Tolerant Near Isogenic Lines of a Mega Basmati Rice Variety, "Pusa Basmati 1121".
AHAS
Basmati rice
DSR
Foreground and background selection
Grain and cooking quality
Herbicide tolerant
Marker assisted backcross breeding
Journal
Rice (New York, N.Y.)
ISSN: 1939-8425
Titre abrégé: Rice (N Y)
Pays: United States
ID NLM: 101503136
Informations de publication
Date de publication:
15 Sep 2020
15 Sep 2020
Historique:
received:
28
05
2020
accepted:
20
08
2020
entrez:
15
9
2020
pubmed:
16
9
2020
medline:
16
9
2020
Statut:
epublish
Résumé
Direct-seeded rice (DSR) is a potential technology for sustainable rice farming as it saves water and labor. However, higher incidence of weed under DSR limits productivity. Therefore, there is a need to develop herbicide tolerant (HT) rice varieties. We used marker assisted backcross breeding (MABB) to transfer a mutant allele of Acetohydroxy acid synthase (AHAS) gene, which confers tolerance to imidazolinone group of herbicides from the donor parent (DP) "Robin" into the genetic background of an elite popular Basmati rice variety, Pusa Basmati 1121 (PB 1121). Foreground selection was done using the AHAS gene linked Simple Sequence Repeat (SSR) marker RM6844 and background selection was performed using 112 genome-wide SSR markers polymorphic between PB 1121 and Robin. Phenotypic selection for agronomic, Basmati grain and cooking quality traits in each generation was carried out to improve the recovery of recurrent parent phenome (RPP). Finally, a set of 12 BC Overall, the present study reports successful development of HT NILs in the genetic background of popular Basmati rice variety, PB 1121 by introgression of mutated AHAS allele. This is the first report on the development of HT Basmati rice. Superior NILs are being evaluated in the national Basmati trials, the release of which is likely to provide a viable option for the adoption of DSR technology in Basmati rice cultivation.
Sections du résumé
BACKGROUND
BACKGROUND
Direct-seeded rice (DSR) is a potential technology for sustainable rice farming as it saves water and labor. However, higher incidence of weed under DSR limits productivity. Therefore, there is a need to develop herbicide tolerant (HT) rice varieties.
RESULTS
RESULTS
We used marker assisted backcross breeding (MABB) to transfer a mutant allele of Acetohydroxy acid synthase (AHAS) gene, which confers tolerance to imidazolinone group of herbicides from the donor parent (DP) "Robin" into the genetic background of an elite popular Basmati rice variety, Pusa Basmati 1121 (PB 1121). Foreground selection was done using the AHAS gene linked Simple Sequence Repeat (SSR) marker RM6844 and background selection was performed using 112 genome-wide SSR markers polymorphic between PB 1121 and Robin. Phenotypic selection for agronomic, Basmati grain and cooking quality traits in each generation was carried out to improve the recovery of recurrent parent phenome (RPP). Finally, a set of 12 BC
CONCLUSION
CONCLUSIONS
Overall, the present study reports successful development of HT NILs in the genetic background of popular Basmati rice variety, PB 1121 by introgression of mutated AHAS allele. This is the first report on the development of HT Basmati rice. Superior NILs are being evaluated in the national Basmati trials, the release of which is likely to provide a viable option for the adoption of DSR technology in Basmati rice cultivation.
Identifiants
pubmed: 32930909
doi: 10.1186/s12284-020-00423-2
pii: 10.1186/s12284-020-00423-2
pmc: PMC7492307
doi:
Types de publication
Journal Article
Langues
eng
Pagination
68Subventions
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : (BT/PR 10787/AGIII/103/883/2014)
Références
Rice (N Y). 2018 Apr 9;11(1):19
pubmed: 29629488
Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1091-E1100
pubmed: 28137884
Plant Cell. 2008 Jul;20(7):1850-61
pubmed: 18599581
AoB Plants. 2012;2012:pls029
pubmed: 23125910
Amino Acids. 2006 Mar;30(2):195-204
pubmed: 16547651
Pest Manag Sci. 2014 Sep;70(9):1340-50
pubmed: 24338926
Plant Cell. 1995 Jul;7(7):935-944
pubmed: 12242394
Pest Manag Sci. 2005 Mar;61(3):246-57
pubmed: 15627242
Rice (N Y). 2017 Dec;10(1):10
pubmed: 28378144
Front Plant Sci. 2017 Jan 26;8:41
pubmed: 28184228
Annu Rev Plant Biol. 2010;61:317-47
pubmed: 20192743
Plant Sci. 2016 Jan;242:330-341
pubmed: 26566849
Plant Biotechnol J. 2005 May;3(3):363-70
pubmed: 17129318
Sci Rep. 2016 Jul 11;6:29188
pubmed: 27403778
Int J Genomics. 2018 Feb 11;2018:8319879
pubmed: 29785398
Theor Appl Genet. 2015 Jul;128(7):1243-59
pubmed: 25869921
Planta. 2018 Mar;247(3):693-703
pubmed: 29170911