A serial multiparametric quantitative magnetic resonance imaging study to assess proteoglycan depletion of human articular cartilage and its effects on functionality.
Aged
Aged, 80 and over
Arthroplasty, Replacement, Knee
Biomechanical Phenomena
Cartilage, Articular
/ metabolism
Collagen
/ metabolism
Female
Humans
Image Processing, Computer-Assisted
/ methods
Knee Joint
/ metabolism
Magnetic Resonance Imaging
/ methods
Male
Middle Aged
Osteoarthritis, Knee
/ metabolism
Proteoglycans
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 09 2020
15 09 2020
Historique:
received:
29
05
2020
accepted:
21
08
2020
entrez:
16
9
2020
pubmed:
17
9
2020
medline:
16
12
2020
Statut:
epublish
Résumé
Water, collagen, and proteoglycans determine articular cartilage functionality. If altered, susceptibility to premature degeneration is increased. This study investigated the effects of enzymatic proteoglycan depletion on cartilage functionality as assessed by advanced Magnetic Resonance Imaging (MRI) techniques under standardized loading. Lateral femoral condylar cartilage-bone samples from patients undergoing knee replacement (n = 29) were serially imaged by Proton Density-weighted and T1, T1ρ, T2, and T2* mapping sequences on a clinical 3.0 T MRI scanner (Achieva, Philips). Using pressure-controlled indentation loading, samples were imaged unloaded and quasi-statically loaded to 15.1 N and 28.6 N, and both before and after exposure to low-concentrated (LT, 0.1 mg/mL, n = 10) or high-concentrated trypsin (HT, 1.0 mg/mL, n = 10). Controls were not treated (n = 9). Responses to loading were assessed for the entire sample and regionally, i.e. sub- and peri-pistonally, and zonally, i.e. upper and lower sample halves. Trypsin effects were quantified as relative changes (Δ), analysed using appropriate statistical tests, and referenced histologically. Histological proteoglycan depletion was reflected by significant sub-pistonal decreases in T1 (p = 0.003) and T2 (p = 0.008) after HT exposure. Loading-induced changes in T1ρ and T2* were not related. In conclusion, proteoglycan depletion alters cartilage functionality and may be assessed using serial T1 and T2 mapping under loading.
Identifiants
pubmed: 32934341
doi: 10.1038/s41598-020-72208-y
pii: 10.1038/s41598-020-72208-y
pmc: PMC7492285
doi:
Substances chimiques
Proteoglycans
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15106Références
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