Sympathetic innervation of inguinal white adipose tissue in the mouse.
iDISCO
imaging
pseudorabies virus
sympathetic chain ganglia
tissue clearing
transsynaptic retrograde tracing
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
01 05 2021
01 05 2021
Historique:
revised:
08
09
2020
received:
21
05
2020
accepted:
09
09
2020
pubmed:
17
9
2020
medline:
12
1
2022
entrez:
16
9
2020
Statut:
ppublish
Résumé
Adipose tissue plays an important role in metabolic homeostasis and its prominent role as endocrine organ is now well recognized. Adipose tissue is controlled via the sympathetic nervous system (SNS). New viral, molecular-genetic tools will soon allow a more detailed study of adipose tissue innervation in metabolic function, yet, the precise anatomical extent of preganglionic and postganglionic inputs to the inguinal white adipose tissue (iWAT) is limited. Furthermore, several viral, molecular-genetic tools will require the use of cre/loxP mouse models, while the available studies on sympathetic iWAT innervation were established in larger species. In this study, we generated a detailed map for the sympathetic innervation of iWAT in male and female mice. We adapted iDISCO tissue clearing to process large, whole-body specimens for an unprecedented view of the natural abdominal SNS. Combined with pseudorabies virus retrograde tracing from the iWAT, we defined the preganglionic and postganglionic sympathetic input to iWAT. We used fluorescence-guided anatomical dissections of sympathetic nerves in reporter mice to further clarify that postganglionic axons connect to iWAT via lateral cutaneous rami (dorsolumbar iWAT portion) and the lumbar plexus (inguinal iWAT portion). Importantly, these rami carry axons that branch to iWAT, as well as axons that travel further to innervate the skin and vasculature, and their functional impact will require consideration in denervation studies. Our study may serve as a comprehensive map for future experiments that employ virally driven neuromodulation techniques to predict anatomy-based viral labeling.
Identifiants
pubmed: 32935348
doi: 10.1002/cne.25031
pmc: PMC7960575
mid: NIHMS1628408
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1465-1485Subventions
Organisme : NIH HHS
ID : OT2 OD023864
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK072476
Pays : United States
Organisme : NIGMS NIH HHS
ID : P30 GM118430
Pays : United States
Informations de copyright
© 2020 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals LLC.
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