Terminal complement complex formation is associated with intervertebral disc degeneration.
Back pain
Complement system
Inflammation
Innate immunity
Scoliosis
Journal
European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
ISSN: 1432-0932
Titre abrégé: Eur Spine J
Pays: Germany
ID NLM: 9301980
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
01
04
2020
accepted:
03
09
2020
revised:
16
06
2020
pubmed:
17
9
2020
medline:
24
6
2021
entrez:
16
9
2020
Statut:
ppublish
Résumé
The complement system is a crucial part of innate immunity. Recent work demonstrated an unexpected contribution to tissue homeostasis and degeneration. This study investigated for the first time, in human disc tissues, the deposition profile of the complement activation product terminal complement complex (TCC), an inflammatory trigger and inducer of cell lysis, and its inhibitor CD59, and their correlation with the degree of disc degeneration (DD). Disc biopsies were collected from patients diagnosed with DD (n = 39, age 63 ± 12) and adolescent idiopathic scoliosis (AIS, n = 10, age 17 ± 4) and compared with discs from healthy Young (n = 11, age 7 ± 7) and Elder (n = 10, age 65 ± 15) donors. Immunohistochemical detection of TCC and CD59 in nucleus pulposus (NP), annulus fibrosus (AF) and endplate (EP) was correlated with age, Pfirrmann grade and Modic changes. Higher percentage of TCC+ cells was detected in the NP and EP of DD compared to Elder (P < 0.05), and in the EP of Young versus Elder (P < 0.001). In DD, TCC deposition was positively correlated with Pfirrmann grade, but not with Modic changes, whereas for Young donors, a negative correlation was found with age, indicating TCC's involvement not only in DD, but also in early stages of skeletal development. Higher CD59 positivity was found in AIS and DD groups compared to Young (P < 0.05), and it was negatively correlated with the age of the patients. TCC deposition positively correlated with the degree of disc degeneration. A functional relevance of TCC may exist in DD, representing a potential target for new therapeutics.
Identifiants
pubmed: 32936402
doi: 10.1007/s00586-020-06592-4
pii: 10.1007/s00586-020-06592-4
doi:
Substances chimiques
Complement Membrane Attack Complex
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-226Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : NE 549/6-1
Organisme : Deutsche Forschungsgemeinschaft
ID : BR 919/12-1
Organisme : Universität Ulm
ID : L.SBN.0157
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