Cell-Type- and Brain-Region-Resolved Mouse Brain Lipidome.
aging
central nervous system
glia
lipidomics
lipids
neurons
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
15 09 2020
15 09 2020
Historique:
received:
18
11
2019
revised:
01
07
2020
accepted:
20
08
2020
entrez:
16
9
2020
pubmed:
17
9
2020
medline:
5
5
2021
Statut:
ppublish
Résumé
Gene and protein expression data provide useful resources for understanding brain function, but little is known about the lipid composition of the brain. Here, we perform quantitative shotgun lipidomics, which enables a cell-type-resolved assessment of the mouse brain lipid composition. We quantify around 700 lipid species and evaluate lipid features including fatty acyl chain length, hydroxylation, and number of acyl chain double bonds, thereby identifying cell-type- and brain-region-specific lipid profiles in adult mice, as well as in aged mice, in apolipoprotein-E-deficient mice, in a model of Alzheimer's disease, and in mice fed different diets. We also integrate lipid with protein expression profiles to predict lipid pathways enriched in specific cell types, such as fatty acid β-oxidation in astrocytes and sphingolipid metabolism in microglia. This resource complements existing brain atlases of gene and protein expression and may be useful for understanding the role of lipids in brain function.
Identifiants
pubmed: 32937123
pii: S2211-1247(20)31121-9
doi: 10.1016/j.celrep.2020.108132
pii:
doi:
Substances chimiques
Amyloid beta-Peptides
0
Apolipoproteins E
0
Lipids
0
Presenilin-1
0
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108132Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests M.A.S. and C.K. are employees and shareholders of Lipotype.