Crystal structures of SARS-CoV-2 ADP-ribose phosphatase: from the apo form to ligand complexes.

ADP-ribose phosphatase domain ADP-ribosylation ADRP COVID-19 Mac1 Nsp3 SARS-CoV-2 crystal structure macrodomain

Journal

IUCrJ
ISSN: 2052-2525
Titre abrégé: IUCrJ
Pays: England
ID NLM: 101623101

Informations de publication

Date de publication:
01 Sep 2020
Historique:
received: 22 05 2020
accepted: 15 07 2020
entrez: 17 9 2020
pubmed: 18 9 2020
medline: 18 9 2020
Statut: epublish

Résumé

Among 15 nonstructural proteins (Nsps), the newly emerging Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) encodes a large, multidomain Nsp3. One of its units is the ADP-ribose phosphatase domain (ADRP; also known as the macrodomain, MacroD), which is believed to interfere with the host immune response. Such a function appears to be linked to the ability of the protein to remove ADP-ribose from ADP-ribosylated proteins and RNA, yet the precise role and molecular targets of the enzyme remain unknown. Here, five high-resolution (1.07-2.01 Å) crystal structures corresponding to the apo form of the protein and its complexes with 2-(

Identifiants

pubmed: 32939273
doi: 10.1107/S2052252520009653
pii: lz5040
pmc: PMC7467174
doi:

Types de publication

Journal Article

Langues

eng

Pagination

814-824

Informations de copyright

© Karolina Michalska et al. 2020.

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Auteurs

Karolina Michalska (K)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

Youngchang Kim (Y)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

Robert Jedrzejczak (R)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

Natalia I Maltseva (NI)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

Lucy Stols (L)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.

Michael Endres (M)

Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.

Andrzej Joachimiak (A)

Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, IL 60667, USA.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439, USA.
Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60367, USA.

Classifications MeSH