A whole-brain connectivity map of mouse insular cortex.
AAV tracing
insular cortex
mouse
neuroscience
rabies virus tracing
whole-brain connectivity
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
17 09 2020
17 09 2020
Historique:
received:
29
01
2020
accepted:
16
09
2020
pubmed:
18
9
2020
medline:
24
2
2021
entrez:
17
9
2020
Statut:
epublish
Résumé
The insular cortex (IC) plays key roles in emotional and regulatory brain functions and is affected across psychiatric diseases. However, the brain-wide connections of the mouse IC have not been comprehensively mapped. Here, we traced the whole-brain inputs and outputs of the mouse IC across its rostro-caudal extent. We employed cell-type-specific monosynaptic rabies virus tracings to characterize afferent connections onto either excitatory or inhibitory IC neurons, and adeno-associated viral tracings to label excitatory efferent axons. While the connectivity between the IC and other cortical regions was highly bidirectional, the IC connectivity with subcortical structures was often unidirectional, revealing prominent cortical-to-subcortical or subcortical-to-cortical pathways. The posterior and medial IC exhibited resembling connectivity patterns, while the anterior IC connectivity was distinct, suggesting two major functional compartments. Our results provide insights into the anatomical architecture of the mouse IC and thus a structural basis to guide investigations into its complex functions.
Identifiants
pubmed: 32940600
doi: 10.7554/eLife.55585
pii: 55585
pmc: PMC7538160
doi:
pii:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SPP1665
Pays : International
Organisme : Horizon 2020 Framework Programme
ID : ERC-2017-STG 758448
Pays : International
Organisme : Agence Nationale de la Recherche
ID : ANR-17-CE37-0021
Pays : International
Informations de copyright
© 2020, Gehrlach et al.
Déclaration de conflit d'intérêts
DG, CW, TG, EC, AK, AH, KC, NG No competing interests declared
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