Localized Cell-Surface Sampling of a Secreted Factor Using Cell-Targeting Beads.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
20 10 2020
20 10 2020
Historique:
pubmed:
18
9
2020
medline:
18
2
2021
entrez:
17
9
2020
Statut:
ppublish
Résumé
Intercellular communication through the secretion of soluble factors plays a vital role in a wide range of biological processes (e.g., homeostasis, immune response), yet identification and quantification of many of these factors can be challenging due to their degradation or sequestration in cell culture media prior to analysis. Here, we present a customizable bead-based system capable of simultaneously binding to live cells (through antibody-mediated cell tethering) and capturing cell-secreted molecules. Our functionalized beads capture secreted molecules (e.g., hepatocyte growth factor secreted by fibroblasts) that are diminished when sampled via traditional supernatant analysis techniques (
Identifiants
pubmed: 32941013
doi: 10.1021/acs.analchem.0c02578
pmc: PMC8259888
mid: NIHMS1693833
doi:
Substances chimiques
Antibodies
0
Antigens, Surface
0
Culture Media
0
Fluoresceins
0
Thy-1 Antigens
0
5-chloromethylfluorescein
136832-63-8
Hepatocyte Growth Factor
67256-21-7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
13634-13640Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM128648
Pays : United States
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