Mitochondria transfer from tumor-activated stromal cells (TASC) to primary Glioblastoma cells.
Mesenchymal stromal cells
Mitochondria
Primary GBM cultures
Tunneling nanotubes
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
26 11 2020
26 11 2020
Historique:
received:
26
08
2020
accepted:
26
08
2020
pubmed:
19
9
2020
medline:
19
3
2021
entrez:
18
9
2020
Statut:
ppublish
Résumé
The tumor microenvironment (TME) controls many aspects of cancer development but little is known about its effect in Glioblastoma (GBM), the main brain tumor in adults. Tumor-activated stromal cell (TASC) population, a component of TME in GBM, was induced in vitro by incubation of MSCs with culture media conditioned by primary cultures of GBM under 3D/organoid conditions. We observed mitochondrial transfer by Tunneling Nanotubes (TNT), extracellular vesicles (EV) and cannibalism from the TASC to GBM and analyzed its effect on both proliferation and survival. We created primary cultures of GBM or TASC in which we have eliminated mitochondrial DNA [Rho 0 (ρ
Identifiants
pubmed: 32943183
pii: S0006-291X(20)31699-5
doi: 10.1016/j.bbrc.2020.08.101
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
139-147Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interest.