Endothelial dysfunction in small arteries and early signs of atherosclerosis in ApoE knockout rats.
Animals
Aorta
/ metabolism
Aortic Diseases
/ metabolism
Apolipoproteins E
/ metabolism
Atherosclerosis
/ blood
Cholesterol, LDL
/ blood
Endothelium, Vascular
/ metabolism
Lung
/ metabolism
Macrophages
/ metabolism
Male
Mesenteric Arteries
/ metabolism
Myocytes, Smooth Muscle
/ metabolism
Prostaglandin-Endoperoxide Synthases
/ metabolism
Rats
Rats, Sprague-Dawley
Triglycerides
/ blood
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 09 2020
17 09 2020
Historique:
received:
19
02
2020
accepted:
17
08
2020
entrez:
18
9
2020
pubmed:
19
9
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Endothelial dysfunction is recognized as a major contributor to atherosclerosis and has been suggested to be evident far before plaque formation. Endothelial dysfunction in small resistance arteries has been suggested to initiate long before changes in conduit arteries. In this study, we address early changes in endothelial function of atherosclerosis prone rats. Male ApoE knockout (KO) rats (11- to 13-weeks-old) were subjected to either a Western or standard diet. The diet intervention continued for a period of 20-24 weeks. Endothelial function of pulmonary and mesenteric arteries was examined in vitro using an isometric myograph. We found that Western diet decreased the contribution of cyclooxygenase (COX) to control the vascular tone of both pulmonary and mesenteric arteries. These changes were associated with early stage atherosclerosis and elevated level of plasma total cholesterol, LDL and triglyceride in ApoE KO rats. Chondroid-transformed smooth muscle cells, calcifications, macrophages accumulation and foam cells were also observed in the aortic arch from ApoE KO rats fed Western diet. The ApoE KO rats are a new model to study endothelial dysfunction during the earlier stages of atherosclerosis and could help us improve preclinical drug development.
Identifiants
pubmed: 32943715
doi: 10.1038/s41598-020-72338-3
pii: 10.1038/s41598-020-72338-3
pmc: PMC7499202
doi:
Substances chimiques
Apolipoproteins E
0
Cholesterol, LDL
0
Triglycerides
0
Prostaglandin-Endoperoxide Synthases
EC 1.14.99.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15296Références
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