A combination of two human monoclonal antibodies cures symptomatic rabies.


Journal

EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: England
ID NLM: 101487380

Informations de publication

Date de publication:
06 11 2020
Historique:
received: 29 04 2020
revised: 26 08 2020
accepted: 27 08 2020
pubmed: 19 9 2020
medline: 19 8 2021
entrez: 18 9 2020
Statut: ppublish

Résumé

Rabies is a neglected disease caused by a neurotropic Lyssavirus, transmitted to humans predominantly by the bite of infected dogs. Rabies is preventable with vaccines or proper post-exposure prophylaxis (PEP), but it still causes about 60,000 deaths every year. No cure exists after the onset of clinical signs, and the case-fatality rate approaches 100% even with advanced supportive care. Here, we report that a combination of two potent neutralizing human monoclonal antibodies directed against the viral envelope glycoprotein cures symptomatic rabid mice. Treatment efficacy requires the concomitant administration of antibodies in the periphery and in the central nervous system through intracerebroventricular infusion. After such treatment, recovered mice presented good clinical condition, viral loads were undetectable, and the brain inflammatory profile was almost normal. Our findings provide the unprecedented proof of concept of an antibody-based therapeutic approach for symptomatic rabies.

Identifiants

pubmed: 32945125
doi: 10.15252/emmm.202012628
pmc: PMC7645379
doi:

Substances chimiques

Antibodies, Monoclonal 0
Antibodies, Neutralizing 0
Antibodies, Viral 0
Rabies Vaccines 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e12628

Subventions

Organisme : INFECT-ERA 2016
ID : INFECT-ERA 2016 ToRRENT
Organisme : Agence Nationale de la Recherche (ANR)
ID : 16-IFEC-0006-01
Organisme : Ministero della Salute (Ministry of Health, Italy)
ID : IZSVe RC 08/09
Organisme : VIR Biotechnology

Informations de copyright

© 2020 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Guilherme Dias de Melo (GD)

Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France.

Florian Sonthonnax (F)

Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France.
Sorbonne-Paris Cité, Cellule Pasteur, Université Paris-Diderot, Paris, France.

Gabriel Lepousez (G)

Perception and Memory Unit, Institut Pasteur, Paris, France.

Grégory Jouvion (G)

Experimental Neuropathology Unit, Institut Pasteur, Paris, France.
INSERM, Pathophysiology of Pediatric Genetic Diseases, Sorbonne Université, Hôpital Armand-Trousseau, UF Génétique Moléculaire, Assistance Publique-Hôpitaux de Paris, Paris, France.

Andrea Minola (A)

Humabs BioMed SA, a subsidiary of Vir Biotechnology, Bellinzona, Switzerland.

Fabrizia Zatta (F)

Humabs BioMed SA, a subsidiary of Vir Biotechnology, Bellinzona, Switzerland.

Florence Larrous (F)

Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France.

Lauriane Kergoat (L)

Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France.

Camille Mazo (C)

Perception and Memory Unit, Institut Pasteur, Paris, France.

Carine Moigneu (C)

Perception and Memory Unit, Institut Pasteur, Paris, France.

Roberta Aiello (R)

Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy.

Angela Salomoni (A)

Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy.

Elise Brisebard (E)

Experimental Neuropathology Unit, Institut Pasteur, Paris, France.
Laboratoire d'Histopathologie, VetAgro-Sup, Université de Lyon, Lyon, France.

Paola De Benedictis (P)

Istituto Zooprofilattico Sperimentale delle Venezie, Padua, Italy.

Davide Corti (D)

Humabs BioMed SA, a subsidiary of Vir Biotechnology, Bellinzona, Switzerland.

Hervé Bourhy (H)

Lyssavirus Epidemiology and Neuropathology Unit, Institut Pasteur, Paris, France.

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