A comprehensive model for the diffusion and hybridization processes of nucleic acid probes in fluorescence in situ hybridization.
FISH
cellular barriers
diffusion
molecular probes
nucleic acid mimics
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
04
03
2020
revised:
02
06
2020
accepted:
05
06
2020
entrez:
18
9
2020
pubmed:
19
9
2020
medline:
10
8
2021
Statut:
ppublish
Résumé
Fluorescence in situ hybridization (FISH) has been extensively used in the past decades for the detection and localization of microorganisms. However, a mechanistic approach of the whole FISH process is still missing, and the main limiting steps for the hybridization to occur remain unclear. In here, FISH is approached as a particular case of a diffusion-reaction kinetics, where molecular probes (MPs) move from the hybridization solution to the target RNA site within the cells. Based on literature models, the characteristic times taken by different MPs to diffuse across multiple cellular barriers, as well as the reaction time associated with the formation of the duplex molecular probe-RNA, were estimated. Structural and size differences at the membrane level of bacterial and animal cells were considered. For bacterial cells, the limiting step for diffusion is likely to be the peptidoglycan layer (characteristic time of 7.94 × 10
Substances chimiques
Fluorescent Dyes
0
Nucleic Acid Probes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
3212-3223Subventions
Organisme : Fundação para a Ciência e a Tecnologia
ID : POCI-01-0145-FEDER-029961
Pays : International
Organisme : Fundação para a Ciência e a Tecnologia
ID : POCI-01-0145-FEDER-031011
Pays : International
Organisme : Fundação para a Ciência e a Tecnologia
ID : SFRH/BD/143491/2019
Pays : International
Organisme : Fundação para a Ciência e a Tecnologia
ID : SFRH/BDE/51909/2012
Pays : International
Organisme : Fundação para a Ciência e a Tecnologia
ID : UIDB/00511/2020
Pays : International
Informations de copyright
© 2020 Wiley Periodicals LLC.
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