Downregulation of miR-27b promotes skin wound healing in a rat model of scald burn by promoting fibroblast proliferation.
deep second-degree scald burn
fibroblasts
microRNA-27b
wound healing
Journal
Experimental and therapeutic medicine
ISSN: 1792-0981
Titre abrégé: Exp Ther Med
Pays: Greece
ID NLM: 101531947
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
09
09
2019
accepted:
29
04
2020
entrez:
21
9
2020
pubmed:
22
9
2020
medline:
22
9
2020
Statut:
ppublish
Résumé
The aim of the present study was to investigate the effect and mechanism of action of microRNA (miR)-27b on skin wound healing in rats with deep second-degree scald burns and in BJ human skin fibroblast cells. Rat models with deep second-degree scald burns were constructed and injected with miR-27b mimics and inhibitors at the wound site daily for 21 days. Healing of burned skin tissues was observed at 0, 3, 7, 14 and 21 days following modeling. H&E and Masson staining were used to observe the pathological structure and degree of collagen fibers in the burned skin tissues. The effects of miR-27b on BJ cell proliferation and migration were determined by MTT and scratch assays. Matrix metalloproteinase-1 (MMP-1), α-smooth muscle actin (α-SMA), collagen I and collagen III expression in rat skin tissues and BJ cells were measured via reverse transcription-quantitative PCR and western blot analysis. The results of the
Identifiants
pubmed: 32952653
doi: 10.3892/etm.2020.9191
pii: ETM-0-0-09191
pmc: PMC7485298
doi:
Types de publication
Journal Article
Langues
eng
Pagination
63Informations de copyright
Copyright: © Bi et al.
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