CAR-T Cells Targeting Epstein-Barr Virus gp350 Validated in a Humanized Mouse Model of EBV Infection and Lymphoproliferative Disease.

CAR-T cells EBV PTLD adoptive T cell therapy gp350 humanized mice lymphoma lymphoproliferation lytic infection transplantation

Journal

Molecular therapy oncolytics
ISSN: 2372-7705
Titre abrégé: Mol Ther Oncolytics
Pays: United States
ID NLM: 101666776

Informations de publication

Date de publication:
25 Sep 2020
Historique:
received: 21 05 2020
accepted: 06 08 2020
entrez: 21 9 2020
pubmed: 22 9 2020
medline: 22 9 2020
Statut: epublish

Résumé

Epstein-Barr virus (EBV) is a latent and oncogenic human herpesvirus. Lytic viral protein expression plays an important role in EBV-associated malignancies. The EBV envelope glycoprotein 350 (gp350) is expressed abundantly during EBV lytic reactivation and sporadically on the surface of latently infected cells. Here we tested T cells expressing gp350-specific chimeric antigen receptors (CARs) containing scFvs derived from two novel gp350-binding, highly neutralizing monoclonal antibodies. The scFvs were fused to CD28/CD3ζ signaling domains in a retroviral vector. The produced gp350CAR-T cells specifically recognized and killed gp350

Identifiants

pubmed: 32953984
doi: 10.1016/j.omto.2020.08.005
pii: S2372-7705(20)30122-4
pmc: PMC7479496
doi:

Types de publication

Journal Article

Langues

eng

Pagination

504-524

Informations de copyright

© 2020 The Author(s).

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Auteurs

Constanze Slabik (C)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Maja Kalbarczyk (M)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Simon Danisch (S)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Reinhard Zeidler (R)

Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, 81377 Munich, Germany.
Department of Otorhinolaryngology, Klinikum der Universität München, Marchioninistr. 15, 81377 Munich, Germany.
German Centre for Infection Research (DZIF), Partner Site Munich, 81377 Munich, Germany.

Frank Klawonn (F)

Biostatistics Group, Helmholtz Centre for Infection Research, 38124 Braunschweig, Germany.
Institute for Information Engineering, Ostfalia University, 38302 Wolfenbuettel, Germany.

Valery Volk (V)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.
Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.

Nicole Krönke (N)

Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.

Friedrich Feuerhake (F)

Institute of Pathology, Hannover Medical School, 30625 Hannover, Germany.
Institute for Neuropathology, University Clinic Freiburg, 79106 Freiburg, Germany.

Constanca Ferreira de Figueiredo (C)

Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, 30625 Hannover, Germany.

Rainer Blasczyk (R)

Institute of Transfusion Medicine and Transplant Engineering, Hannover Medical School, 30625 Hannover, Germany.

Henning Olbrich (H)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Sebastian J Theobald (SJ)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Andreas Schneider (A)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Arnold Ganser (A)

Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.

Constantin von Kaisenberg (C)

Department of Obstetrics, Gynecology and Reproductive Medicine, Hannover Medical School, 30625 Hannover, Germany.

Stefan Lienenklaus (S)

Institute for Laboratory Animal Science, Hannover Medical School, 30625 Hannover, Germany.

Andre Bleich (A)

Institute for Laboratory Animal Science, Hannover Medical School, 30625 Hannover, Germany.

Wolfgang Hammerschmidt (W)

Research Unit Gene Vectors, Helmholtz Zentrum München, German Research Center for Environmental Health, 81377 Munich, Germany.
German Centre for Infection Research (DZIF), Partner Site Munich, 81377 Munich, Germany.

Renata Stripecke (R)

Laboratory of Regenerative Immune Therapies Applied, Hannover Medical School, 30625 Hannover, Germany.
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, 30625 Hannover, Germany.
German Centre for Infection Research (DZIF), Partner Site Hannover, 30625 Hannover, Germany.

Classifications MeSH