Characteristics of the Mucosal Surface on Scanning Electron Microscopy in Patients with Remitting Ulcerative Colitis.
Biopsy
/ methods
Cecum
/ diagnostic imaging
Colitis, Ulcerative
/ pathology
Colonoscopy
/ methods
Female
Humans
Intestinal Mucosa
/ diagnostic imaging
Male
Microscopy, Electron, Scanning
/ methods
Middle Aged
Outcome Assessment, Health Care
/ methods
Prognosis
Rectum
/ diagnostic imaging
Remission Induction
Inflammatory bowel disease
Intestinal epithelium
Scanning electron microscopy
Ulcerative colitis
Journal
Digestive diseases and sciences
ISSN: 1573-2568
Titre abrégé: Dig Dis Sci
Pays: United States
ID NLM: 7902782
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
11
06
2020
accepted:
07
09
2020
pubmed:
22
9
2020
medline:
30
9
2021
entrez:
21
9
2020
Statut:
ppublish
Résumé
Ulcerative colitis (UC) is characterized by chronic intestinal epithelial damage, and previous studies have evaluated the epithelial structure of patients with active UC using electron microscopy. This study aimed to assess the intestinal epithelial structure using scanning electron microscopy (SEM) and the features of patients with UC who are in remission. In total, eight healthy controls and 20 patients with UC were enrolled, and colonic tissue samples from the cecum and rectum were collected. Then, we compared the epithelial surface structure on SEM between patients with UC who are in clinical remission and healthy controls. In healthy controls, the colonic surface comprises small lobes (termed units), with one crypt located in the middle of each unit. In patients with UC, we found irregular unit and crypt mouth size, double crypt sign (> 1 crypt per unit), and lower number of small vesicles in the intestinal epithelial cells. Compared with healthy controls, patients with UC often presented with irregular unit size, double crypt sign, and irregular crypt mouth size in the rectum. The small vesicles were observed less frequently in patients with UC than in healthy controls. SEM revealed a unique epithelial structure in patients with UC who are in remission.
Sections du résumé
BACKGROUND
Ulcerative colitis (UC) is characterized by chronic intestinal epithelial damage, and previous studies have evaluated the epithelial structure of patients with active UC using electron microscopy.
AIMS
This study aimed to assess the intestinal epithelial structure using scanning electron microscopy (SEM) and the features of patients with UC who are in remission.
METHODS
In total, eight healthy controls and 20 patients with UC were enrolled, and colonic tissue samples from the cecum and rectum were collected. Then, we compared the epithelial surface structure on SEM between patients with UC who are in clinical remission and healthy controls.
RESULTS
In healthy controls, the colonic surface comprises small lobes (termed units), with one crypt located in the middle of each unit. In patients with UC, we found irregular unit and crypt mouth size, double crypt sign (> 1 crypt per unit), and lower number of small vesicles in the intestinal epithelial cells. Compared with healthy controls, patients with UC often presented with irregular unit size, double crypt sign, and irregular crypt mouth size in the rectum. The small vesicles were observed less frequently in patients with UC than in healthy controls.
CONCLUSIONS
SEM revealed a unique epithelial structure in patients with UC who are in remission.
Identifiants
pubmed: 32955688
doi: 10.1007/s10620-020-06609-4
pii: 10.1007/s10620-020-06609-4
doi:
Banques de données
UMIN-CTR
['UMIN000027697']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3141-3148Informations de copyright
© 2020. Springer Science+Business Media, LLC, part of Springer Nature.
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