Cell Banking of hiPSCs: A Practical Guide to Cryopreservation and Quality Control in Basic Research.
cell banking
hiPSC
master cell bank
stem cell quality
Journal
Current protocols in stem cell biology
ISSN: 1938-8969
Titre abrégé: Curr Protoc Stem Cell Biol
Pays: United States
ID NLM: 101470226
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
entrez:
21
9
2020
pubmed:
22
9
2020
medline:
11
8
2021
Statut:
ppublish
Résumé
The reproducibility of stem cell research relies on the constant availability of quality-controlled cells. As the quality of human induced pluripotent stem cells (hiPSCs) can deteriorate in the course of a few passages, cell banking is key to achieve consistent results and low batch-to-batch variation. Here, we provide a cost-efficient route to generate master and working cell banks for basic research projects. In addition, we describe minimal protocols for quality assurance including tests for sterility, viability, pluripotency, and genetic integrity. © 2020 The Authors. Basic Protocol 1: Expansion of hiPSCs Basic Protocol 2: Cell banking of hiPSCs Support Protocol 1: Pluripotency assessment by flow cytometry Support Protocol 2: Thawing control: Viability and sterility Support Protocol 3: Potency, viral clearance, and pluripotency: Spontaneous differentiation and qRT-PCR Support Protocol 4: Identity: Short tandem repeat analysis.
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e127Informations de copyright
© 2020 The Authors.