Ecotoxicogenomic analysis of stress induced on Caenorhabditis elegans in heavy metal contaminated soil after nZVI treatment.
Animals
Caenorhabditis elegans
/ drug effects
Caenorhabditis elegans Proteins
/ genetics
Ecotoxicology
Iron
/ chemistry
Metal Nanoparticles
Metals, Heavy
/ analysis
Nanoparticles
/ chemistry
Oxidative Stress
/ drug effects
Soil
/ chemistry
Soil Microbiology
Soil Pollutants
/ analysis
Toxicogenetics
Transcription, Genetic
/ drug effects
C. elegans
Ecotoxicogenomic
Heavy metals
Microarray
Nanoremediation
Soil
Journal
Chemosphere
ISSN: 1879-1298
Titre abrégé: Chemosphere
Pays: England
ID NLM: 0320657
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
28
02
2020
revised:
24
04
2020
accepted:
25
04
2020
entrez:
22
9
2020
pubmed:
23
9
2020
medline:
3
10
2020
Statut:
ppublish
Résumé
Soil contamination by heavy metals (HMs) is an environmental problem, and nanoremediation by using zero-valent iron nanoparticles (nZVI) has attracted increasing interest. We used ecotoxicological test and global transcriptome analysis with DNA microarrays to assess the suitability of C. elegans as a useful bioindicator to evaluate such strategy of nanoremediation in a highly polluted soil with Pb, Cd and Zn. The HMs produced devastating effect on C. elegans. nZVI treatment reversed this deleterious effect up to day 30 after application, but the reduction in the relative toxicity of HMs was lower at day 120. We stablished gene expression profile in C. elegans exposed to the polluted soil, treated and untreated with nZVI. The percentage of differentially expressed genes after treatment decreases with exposure time. After application of nZVI we found decreased toxicity, but increased biosynthesis of defensive enzymes responsive to oxidative stress. At day 14, when a decrease in toxicity has occurred, genes related to specific heavy metal detoxification mechanisms or to response to metal stress, were down regulated: gst-genes, encoding for glutathione-S-transferase, htm-1 (heavy metal tolerance factor), and pgp-5 and pgp-7, related to stress response to metals. At day 120, we found increased HMs toxicity compared to day 14, whereas the transcriptional oxidative and metal-induced responses were attenuated. These findings indicate that the profiled gene expression in C. elegans may be considered as an indicator of stress response that allows a reliable evaluation of the nanoremediation strategy.
Identifiants
pubmed: 32957299
pii: S0045-6535(20)31102-4
doi: 10.1016/j.chemosphere.2020.126909
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Metals, Heavy
0
Soil
0
Soil Pollutants
0
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126909Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.