Metagenomic sequencing of stool samples in Bangladeshi infants: virome association with poliovirus shedding after oral poliovirus vaccination.
Antibodies, Neutralizing
/ immunology
Antibodies, Viral
/ blood
Bangladesh
/ epidemiology
Feces
/ virology
Female
Humans
Immunization Schedule
Infant
Male
Metagenome
/ genetics
Metagenomics
/ methods
Poliomyelitis
/ virology
Poliovirus
/ genetics
Poliovirus Vaccine, Inactivated
/ immunology
Poliovirus Vaccine, Oral
/ immunology
Vaccination
Virome
/ genetics
Virus Shedding
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 09 2020
21 09 2020
Historique:
received:
23
10
2019
accepted:
09
07
2020
entrez:
22
9
2020
pubmed:
23
9
2020
medline:
18
12
2020
Statut:
epublish
Résumé
The potential role of enteric viral infections and the developing infant virome in affecting immune responses to the oral poliovirus vaccine (OPV) is unknown. Here we performed viral metagenomic sequencing on 3 serially collected stool samples from 30 Bangladeshi infants following OPV vaccination and compared findings to stool samples from 16 age-matched infants in the United States (US). In 14 Bangladeshi infants, available post-vaccination serum samples were tested for polio-neutralizing antibodies. The abundance (p = 0.006) and richness (p = 0.013) of the eukaryotic virome increased with age and were higher than seen in age-matched US infants (p < 0.001). In contrast, phage diversity metrics remained stable and were similar to those in US infants. Non-poliovirus eukaryotic virus abundance (3.68 log
Identifiants
pubmed: 32958861
doi: 10.1038/s41598-020-71791-4
pii: 10.1038/s41598-020-71791-4
pmc: PMC7506025
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Poliovirus Vaccine, Inactivated
0
Poliovirus Vaccine, Oral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15392Subventions
Organisme : NICHD NIH HHS
ID : R01 HD088837
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR001084
Pays : United States
Organisme : NIH HHS
ID : R01-HD008837
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR003142
Pays : United States
Organisme : NCRR NIH HHS
ID : UL1 RR025744
Pays : United States
Organisme : NIH HHS
ID : R01501HD063142
Pays : United States
Références
Front Microbiol. 2018 Jun 01;9:1162
pubmed: 29910789
J Infect Dis. 2018 Apr 11;217(9):1395-1398
pubmed: 29300947
Nat Commun. 2018 Nov 14;9(1):4852
pubmed: 30429475
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Nat Med. 2017 Sep;23(9):1080-1085
pubmed: 28759053
Clin Infect Dis. 2016 Jan 15;62(2):150-6
pubmed: 26349548
Virology. 2014 Nov;468-470:556-564
pubmed: 25262473
J Infect Dis. 2019 Apr 8;219(8):1178-1186
pubmed: 30247561
Br Med J. 1976 Apr 3;1(6013):812
pubmed: 1260345
Lab Invest. 2012 Mar;92(3):458-65
pubmed: 22157715
Genome Res. 2019 May;29(5):831-842
pubmed: 30992304
PLoS One. 2018 Aug 16;13(8):e0202054
pubmed: 30114205
J Infect Dis. 2014 Sep 15;210(6):853-64
pubmed: 24688069
PLoS One. 2015 Oct 02;10(10):e0139810
pubmed: 26431175
PLoS One. 2019 Oct 24;14(10):e0223952
pubmed: 31647831
Clin Vaccine Immunol. 2011 Nov;18(11):1889-94
pubmed: 21880850
Microbiome. 2017 Jun 26;5(1):66
pubmed: 28651630
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Curr Protoc Microbiol. 2013;Chapter 15:15H.1.1-15H.1.27
pubmed: 23686830
Vaccine. 2016 Jun 8;34(27):3068-3075
pubmed: 27154394
Nat Med. 2015 Oct;21(10):1228-34
pubmed: 26366711
Trends Microbiol. 2016 Oct;24(10):801-810
pubmed: 27353648
PLoS One. 2015 Nov 23;10(11):e0141723
pubmed: 26599538
Rev Infect Dis. 1991 Sep-Oct;13(5):926-39
pubmed: 1660184
Vaccine. 2012 Apr 27;30 Suppl 1:A30-5
pubmed: 22520134
Future Microbiol. 2018 Jan;13:97-118
pubmed: 29218997
BMC Biol. 2019 Oct 28;17(1):84
pubmed: 31660953
Mol Cell Probes. 1991 Dec;5(6):473-81
pubmed: 1664049
Nature. 2016 Feb 11;530(7589):228-232
pubmed: 26840485
Viruses. 2019 Feb 24;11(2):
pubmed: 30813498
BMC Complement Altern Med. 2016 Feb 02;16:44
pubmed: 26832746
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Genome Res. 2014 Jul;24(7):1180-92
pubmed: 24899342
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638