Pollen morphology and viability of Tillandsia (Bromeliaceae) species by light microscopy and scanning electron microscopy.
LM
SEM
Tillandsioideae
histochemistry
in vitro pollen germination
Journal
Microscopy research and technique
ISSN: 1097-0029
Titre abrégé: Microsc Res Tech
Pays: United States
ID NLM: 9203012
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
09
06
2020
revised:
19
08
2020
accepted:
07
09
2020
pubmed:
23
9
2020
medline:
19
8
2021
entrez:
22
9
2020
Statut:
ppublish
Résumé
Tillandsia is the bromeliad genus containing the largest number of species, with wide geographic dispersion and an important ecological role in the ecosystems. Investigations of pollen morphology are important to support taxonomic and conservation studies as well as to choose promising species for cross-pollination to obtain hybrids. The objective of this study was to evaluate the morphology, production, and viability of pollen grains of 24 Tillandsia species that naturally occur in the state of Bahia, Brazil. Pollen grains were acetolized and analyzed by scanning electron microscopy. The pollen were germinated in vitro in two culture media (BM and BKM) and collected at three floral development stages (pre-anthesis, anthesis and post-anthesis). Pollen viability also was analyzed by staining with Alexander's solution and acetocarmine in three floral development stages. Variations in the ornamentation and size of the pollen were observed among the species studied, with the majority having medium size, while T. polystachia and T. juncea had large grains and the subgenera Diaphoranthema and Phytarrhiza had small grains. The pollen of the majority of species had bilateral symmetry, with a single irregularly shaped colpus, semitectate exine and reticulated-heterobrochate surface. The pollen presented high germination percentage, tube length and viability according to the histochemistry, except for T. recurvata, T. usneoides and T. loliacea. The BKM medium and collection of pollen during anthesis produced the best results for the Tillandsia species. The results can support studies of the taxonomy and conservation of these species, which have great environmental importance and high value for ornamental purposes.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
441-459Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 304269/2018-2
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : Finance Code 001
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : PNPD/ UEFS - 15950830814
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : PNPD/ UFRB - 88882.315208/2019-01
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : PROCAD - 88887.124186/2014-00
Organisme : Fundação de Amparo a Pesquisa do Estado da Bahia
ID : BOL0177/2018
Informations de copyright
© 2020 Wiley Periodicals LLC.
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