Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues.
LC-HRMS
high resolution
lactoyl-CoA
lactyl-CoA
metabolism
Journal
Open biology
ISSN: 2046-2441
Titre abrégé: Open Biol
Pays: England
ID NLM: 101580419
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
entrez:
22
9
2020
pubmed:
23
9
2020
medline:
17
8
2021
Statut:
ppublish
Résumé
Lysine lactoylation is a recently described protein post-translational modification (PTM). However, the biochemical pathways responsible for this acylation remain unclear. Two metabolite-dependent mechanisms have been proposed: enzymatic histone lysine lactoylation derived from lactoyl-coenzyme A (lactoyl-CoA, also termed lactyl-CoA), and non-enzymatic lysine lactoylation resulting from acyl-transfer via lactoyl-glutathione. While the former has precedent in the form of enzyme-catalysed lysine acylation, the lactoyl-CoA metabolite has not been previously quantified in mammalian systems. Here, we use liquid chromatography-high-resolution mass spectrometry (LC-HRMS) together with a synthetic standard to detect and validate the presence of lactoyl-CoA in cell and tissue samples. Conducting a retrospective analysis of data from previously analysed samples revealed the presence of lactoyl-CoA in diverse cell and tissue contexts. In addition, we describe a biosynthetic route to generate
Identifiants
pubmed: 32961073
doi: 10.1098/rsob.200187
pmc: PMC7536085
doi:
Substances chimiques
Acyl Coenzyme A
0
Biomarkers
0
lactoyl-coenzyme A
1926-57-4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
200187Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM007229
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM132261
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK109100
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA228339
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC011488
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA115299
Pays : United States
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