A review of phosphatidate phosphatase assays.
Pah1
diacylglycerol
enzyme assays
lipid metabolism
lipin
nonradioactive assays
radioactive assays
triacylglycerol
Journal
Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
pubmed:
24
9
2020
medline:
26
10
2021
entrez:
23
9
2020
Statut:
ppublish
Résumé
Phosphatidate phosphatase (PAP) catalyzes the penultimate step in the synthesis of triacylglycerol and regulates the synthesis of membrane phospholipids. There is much interest in this enzyme because it controls the cellular levels of its substrate, phosphatidate (PA), and product, DAG; defects in the metabolism of these lipid intermediates are the basis for lipid-based diseases such as obesity, lipodystrophy, and inflammation. The measurement of PAP activity is required for studies aimed at understanding its mechanisms of action, how it is regulated, and for screening its activators and/or inhibitors. Enzyme activity is determined through the use of radioactive and nonradioactive assays that measure the product, DAG, or P
Identifiants
pubmed: 32963036
pii: S0022-2275(20)60018-9
doi: 10.1194/jlr.R120001092
pmc: PMC7707177
pii:
doi:
Substances chimiques
Phosphatidate Phosphatase
EC 3.1.3.4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1556-1564Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM028140
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM050679
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136128
Pays : United States
Organisme : NIGMS NIH HHS
ID : R37 GM028140
Pays : United States
Informations de copyright
Copyright © 2020 Dey et al.
Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
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