Regulation of degenerative spheroids after injury.

Animals Armadillo Domain Proteins / physiology Axons / pathology Axotomy Calcium / metabolism Cytoskeletal Proteins / physiology Female Male Mice Mice, Inbred C57BL Mice, Knockout Nerve Tissue Proteins / physiology Neurodegenerative Diseases / etiology Neurons / metabolism Receptors, Tumor Necrosis Factor / physiology Spheroids, Cellular / pathology Wallerian Degeneration / physiopathology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
22 09 2020
Historique:
received: 14 05 2020
accepted: 05 08 2020
entrez: 23 9 2020
pubmed: 24 9 2020
medline: 16 12 2020
Statut: epublish

Résumé

Neuronal injury leads to rapid, programmed disintegration of axons distal to the site of lesion. Much like other forms of axon degeneration (e.g. developmental pruning, toxic insult from neurodegenerative disorder), Wallerian degeneration associated with injury is preceded by spheroid formation along axons. The mechanisms by which injury leads to formation of spheroids and whether these spheroids have a functional role in degeneration remain elusive. Here, using neonatal mouse primary sympathetic neurons, we investigate the roles of players previously implicated in the progression of Wallerian degeneration in injury-induced spheroid formation. We find that intra-axonal calcium flux is accompanied by actin-Rho dependent growth of calcium rich axonal spheroids that eventually rupture, releasing material to the extracellular space prior to catastrophic axon degeneration. Importantly, after injury, Sarm1

Identifiants

DOI: 10.1038/s41598-020-71906-x PMID: 32963272 PMC: PMC7508847
pubmed: 32963272
doi: 10.1038/s41598-020-71906-x
pii: 10.1038/s41598-020-71906-x
pmc: PMC7508847
doi:

Substances chimiques

Armadillo Domain Proteins 0
Cytoskeletal Proteins 0
Nerve Tissue Proteins 0
Receptors, Tumor Necrosis Factor 0
SARM1 protein, mouse 0
Tnfrsf21 protein, mouse 0
Wld protein, mouse 0
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15472

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS091617
Pays : United States
Organisme : NINDS NIH HHS
ID : R01NS091617
Pays : United States

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Auteurs

Yu Yong (Y)

Department of Biology, University of Virginia, Charlottesville, VA, 22904-4328, USA.

Kanchana Gamage (K)

Amgen, Massachusetts and Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, 02138, USA.

Courtny Cushman (C)

Department of Neuroscience and Biomedical Engineering, University of Virginia, Charlottesville, VA, 22904-4328, USA.

Anthony Spano (A)

Department of Biology, University of Virginia, Charlottesville, VA, 22904-4328, USA.

Christopher Deppmann (C)

Department of Biology, University of Virginia, Charlottesville, VA, 22904-4328, USA. deppmann@virginia.edu.
Department of Neuroscience and Biomedical Engineering, University of Virginia, Charlottesville, VA, 22904-4328, USA. deppmann@virginia.edu.
Department of Cell Biology, University of Virginia, Charlottesville, VA, 22904-4328, USA. deppmann@virginia.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines à domaine armadillo › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Axones › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Procédures de chirurgie opératoire › Procédures de neurochirurgie › Dénervation › Axotomie › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Souris knockout › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Maladies du système nerveux › Maladies neurodégénératives › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Cellules › Neurones › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Récepteurs aux cytokines › Récepteurs aux facteurs de nécrose tumorale › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › Cellules › Cellules cultivées › Sphéroïdes de cellules › Regulation of degenerative spheroids after injury.
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Dégénérescence nerveuse › Dégénérescence wallerienne › Regulation of degenerative spheroids after injury.