A high throughput screening system for studying the effects of applied mechanical forces on reprogramming factor expression.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 09 2020
22 09 2020
Historique:
received:
11
09
2019
accepted:
20
08
2020
entrez:
23
9
2020
pubmed:
24
9
2020
medline:
16
12
2020
Statut:
epublish
Résumé
Mechanical forces are important in the regulation of physiological homeostasis and the development of disease. The application of mechanical forces to cultured cells is often performed using specialized systems that lack the flexibility and throughput of other biological techniques. In this study, we developed a high throughput platform for applying complex dynamic mechanical forces to cultured cells. We validated the system for its ability to accurately apply parallel mechanical stretch in a 96 well plate format in 576 well simultaneously. Using this system, we screened for optimized conditions to stimulate increases in Oct-4 and other transcription factor expression in mouse fibroblasts. Using high throughput mechanobiological screening assays, we identified small molecules that can synergistically enhance the increase in reprograming-related gene expression in mouse fibroblasts when combined with mechanical loading. Taken together, our findings demonstrate a new powerful tool for investigating the mechanobiological mechanisms of disease and performing drug screening in the presence of applied mechanical load.
Identifiants
pubmed: 32963285
doi: 10.1038/s41598-020-72158-5
pii: 10.1038/s41598-020-72158-5
pmc: PMC7508814
doi:
Substances chimiques
Small Molecule Libraries
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
15469Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL141761
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB024147
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB023551
Pays : United States
Organisme : NIH HHS
ID : 1R21EB023551-01
Pays : United States
Organisme : American Heart Association-American Stroke Association
ID : 17IRG33410888
Pays : United States
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