Effects of Concentration on Aggregation of BODIPY-Based Fluorescent Dyes Solution.

Despite significant progress in understanding of dye aggregation, there are still processes that need to be further explored and which can significantly affect aggregation. In this work it was shown that the aggregation of dyes is influenced not only by dye concentration, but also by solvent polarity. It was found that nature, positions and number of fluorescent peaks may be controlled by simultaneous varying of both water fraction and dye concentration. This effect is most pronounced for molecular rotors, which aggregates' geometry may be stabilized in different separate states depending on the aggregation degree. The concentration effect plays a significant role in dye aggregation and should be considered in new studies in order to prevent misinterpretation or to obtain new results in fields of molecular sensing or fine-tuning of fluorescence color. In this paper aggregation caused spectral changes are discussed in line with the dye structure preorganization as the strategy for the fine tuning of practically valuable spectral characteristics.