Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
16
04
2020
accepted:
07
08
2020
entrez:
24
9
2020
pubmed:
25
9
2020
medline:
25
9
2020
Statut:
epublish
Résumé
Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors.
Identifiants
pubmed: 32967835
pii: 6/39/eabc2648
doi: 10.1126/sciadv.abc2648
pmc: PMC7531888
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Références
J Cell Sci. 1997 Sep;110 ( Pt 17):2109-16
pubmed: 9378761
Bioarchitecture. 2012 May 1;2(3):75-87
pubmed: 22880146
Adv Mater. 2019 Jun;31(26):e1808110
pubmed: 30793374
Adv Mater. 2014 Apr 16;26(15):2319-22
pubmed: 24421068
Acc Chem Res. 2014 Jul 15;47(7):2128-40
pubmed: 24911321
Sci Rep. 2016 Feb 05;6:20554
pubmed: 26847712
Proc Natl Acad Sci U S A. 2012 May 1;109(18):6933-8
pubmed: 22509005
Chem Rev. 1998 Jul 30;98(5):1875-1918
pubmed: 11848952
Sci Rep. 2016 Apr 15;6:24264
pubmed: 27080570
J Cell Biol. 2017 Sep 4;216(9):2877-2889
pubmed: 28701425
Angew Chem Int Ed Engl. 2017 Jul 10;56(29):8350-8369
pubmed: 28245083
Sci Rep. 2018 Jan 31;8(1):1963
pubmed: 29386555
J Cell Sci. 2016 Apr 1;129(7):1293-304
pubmed: 27037413
PLoS Comput Biol. 2015 Mar 06;11(3):e1004076
pubmed: 25748431
Nat Mater. 2016 Sep;15(9):961-967
pubmed: 27240108
J Biomech. 2000 Jan;33(1):3-14
pubmed: 10609513
Biophys J. 2017 Aug 22;113(4):770-774
pubmed: 28755755
Adv Mater. 2017 Feb;29(5):
pubmed: 27882610
Biomaterials. 1995 Mar;16(4):297-303
pubmed: 7772669
Science. 2017 May 5;356(6337):
pubmed: 28473537
Sci Rep. 2017 Aug 9;7(1):7660
pubmed: 28794475
Mol Cell Biol. 2008 Sep;28(17):5196-208
pubmed: 18573880
J Cell Sci. 2006 Feb 1;119(Pt 3):508-18
pubmed: 16443749
Science. 1996 Jul 12;273(5272):245-8
pubmed: 8662509
Nat Cell Biol. 2001 May;3(5):466-72
pubmed: 11331874
J Cell Sci. 2017 Jan 1;130(1):51-61
pubmed: 27856508
J Cell Sci. 2012 Jul 1;125(Pt 13):3015-24
pubmed: 22797912
Biophys J. 2014 Jul 1;107(1):146-55
pubmed: 24988349
Biomaterials. 2015 Mar;44:186-94
pubmed: 25617137
Mol Syst Biol. 2017 Sep 27;13(9):941
pubmed: 28954810
Nature. 2011 Jun 08;474(7350):179-83
pubmed: 21654799
Lab Chip. 2016 Aug 16;16(17):3193-203
pubmed: 27440436
Nat Mater. 2014 Jun;13(6):645-52
pubmed: 24633344
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1484-9
pubmed: 12552122
Arch Biochem Biophys. 2006 Dec 15;456(2):224-31
pubmed: 17094935
Nat Chem. 2011 Jan;3(1):34-7
pubmed: 21160514
Nat Rev Mol Cell Biol. 2009 Nov;10(11):778-90
pubmed: 19851336
Nat Commun. 2012 Apr 24;3:792
pubmed: 22531177
Biochem Cell Biol. 1995 Jul-Aug;73(7-8):349-65
pubmed: 8703408
Nat Commun. 2019 Jan 16;10(1):232
pubmed: 30651553
Nat Cell Biol. 2003 Nov;5(11):980-6
pubmed: 14578909
Biomaterials. 2009 Dec;30(35):6702-7
pubmed: 19783300
J Cell Physiol. 1998 Jun;175(3):323-32
pubmed: 9572477
J Biomech. 2011 May 17;44(8):1433-46
pubmed: 21489537
Invest Ophthalmol Vis Sci. 2010 Aug;51(8):3853-63
pubmed: 20220050