Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
23 11 2020
Historique:
received: 31 05 2020
accepted: 17 09 2020
pubmed: 25 9 2020
medline: 26 1 2021
entrez: 24 9 2020
Statut: epublish

Résumé

Influenza virus isolation from clinical samples is critical for the identification and characterization of circulating and emerging viruses. Yet efficient isolation can be difficult. In these studies, we isolated primary swine nasal and tracheal respiratory epithelial cells and immortalized swine nasal epithelial cells (siNEC) and tracheal epithelial cells (siTEC) that retained the abilities to form tight junctions and cilia and to differentiate at the air-liquid interface like primary cells. Critically, both human and swine influenza viruses replicated in the immortalized cells, which generally yielded higher-titer viral isolates from human and swine nasal swabs, supported the replication of isolates that failed to grow in Madin-Darby canine kidney (MDCK) cells, and resulted in fewer dominating mutations during viral passaging than MDCK cells.

Identifiants

pubmed: 32967961
pii: JVI.01091-20
doi: 10.1128/JVI.01091-20
pmc: PMC7925196
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : HHSN272201400006C
Pays : United States

Informations de copyright

Copyright © 2020 American Society for Microbiology.

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Auteurs

Victoria Meliopoulos (V)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA victoria.meliopoulos@stjude.org.

Sean Cherry (S)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Nicholas Wohlgemuth (N)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

Rebekah Honce (R)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
Integrated Program in Biomedical Sciences, College of Graduate Health Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA.

Karen Barnard (K)

Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.

Phillip Gauger (P)

Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.

Todd Davis (T)

Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control, Atlanta, Georgia, USA.

Peter Shult (P)

Wisconsin State Laboratory of Hygiene, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Colin Parrish (C)

Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA.

Stacey Schultz-Cherry (S)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.

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Classifications MeSH