Using next-generation sequencing of microRNAs to identify host and/or pathogen nucleic acid signatures in samples from children with biliary atresia - a pilot study.
biliary atresia
microRNA
next-generation sequencing
Journal
Access microbiology
ISSN: 2516-8290
Titre abrégé: Access Microbiol
Pays: England
ID NLM: 101746927
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
01
2019
accepted:
24
02
2020
entrez:
25
9
2020
pubmed:
26
9
2020
medline:
26
9
2020
Statut:
epublish
Résumé
Biliary atresia (BA) is a progressive disease affecting infants resulting in inflammatory obliteration and fibrosis of the extra- and intra-hepatic biliary tree. BA may be grouped into type 1 isolated; type 2 syndromic, where other congenital malformations may be present; type 3 cystic BA, where there is cyst formation within an otherwise obliterated biliary tree; and cytomegalovirus-associated BA. The cause of BA is unclear, with immune dysregulation, inflammation and infection, particularly with cytomegalovirus (CMV), all implicated. In this study a total of 50/67 samples were tested for CMV DNA using quantitative real-time PCR. Ten liver tissue and 8 bile samples from 10 patients representing the range of BA types were also analysed by next-generation sequencing. CMV DNA was found in 8/50 (16 %) patients and a total of 265 differentially expressed microRNAs were identified. No statistically significant differences between the various types of BA were found. However, differences were identified in the expression patterns of 110 microRNAs in bile and liver tissue samples (
Identifiants
pubmed: 32974591
doi: 10.1099/acmi.0.000127
pii: 000127
pmc: PMC7497833
doi:
Types de publication
Journal Article
Langues
eng
Pagination
acmi000127Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare that there are no conflicts of interest.
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