Vitrification of donkey sperm using straws as an alternative to conventional slow freezing.
cryopreservation
jack
semen
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
10
07
2020
received:
09
05
2020
accepted:
21
07
2020
pubmed:
26
9
2020
medline:
2
2
2022
entrez:
25
9
2020
Statut:
ppublish
Résumé
Cryoprotectant-free vitrification of donkey sperm using 0.25 ml straws has been recently developed, but the obtained results have not been directly compared to conventional slow freezing yet. The aim of this study was to compare sperm quality parameters after cryopreservation using both methods. Semen samples were collected from three Andalusian Donkeys. Semen was centrifuged and pellets resuspended with an extender with glycerol for conventional freezing or the same extender without glycerol, but with sucrose 0.1 mol/L for vitrification. Conventional freezing was performed in nitrogen vapours and thawed in a water bath (30s/37°C). Vitrification was performed in covered 0.25 ml straws plunged directly into liquid nitrogen and warmed in 3 ml of a milk-based extender at 43°C. Total (TM, %) and progressive motility (PM, %) were evaluated by computer-assisted sperm analysis, and plasma membrane (PMI, %) and acrosome (AIS, %) integrities by epifluorescence microscopy. No differences (p > .05) were found between slow freezing and vitrification methods for any of the parameters assessed: TM (58.2 ± 16.1% vs. 52.7 ± 15.6%), PM (44.7 ± 18.2% vs. 44.3 ± 15.0%), PMI (55.4 ± 9.0% vs. 49.2 ± 11.2%) and AIS (38.4 ± 19.6% vs. 45.0 ± 11.0%), respectively. In conclusion, donkey sperm vitrification in straws presented similar sperm quality after thawing in comparison to conventional freezing. Therefore, it could be considered as an alternative to slow freezing regarding the sperm parameters assessed.
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3-6Subventions
Organisme : Ministerio de Educación, Cultura y Deporte
Organisme : Ministerio de Economía y Competitividad
ID : AGL-2013-42726-R
Informations de copyright
© 2020 Blackwell Verlag GmbH.
Références
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