Photocaged Hoechst Enables Subnuclear Visualization and Cell Selective Staining of DNA in vivo.
DNA
Hoechst
labeling
microscopy
zebrafish
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
02 02 2021
02 02 2021
Historique:
received:
13
07
2020
revised:
17
09
2020
pubmed:
26
9
2020
medline:
24
9
2021
entrez:
25
9
2020
Statut:
ppublish
Résumé
Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms.
Identifiants
pubmed: 32974998
doi: 10.1002/cbic.202000465
pmc: PMC7894298
doi:
Substances chimiques
Fluorescent Dyes
0
Luminescent Proteins
0
Recombinant Fusion Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
548-556Informations de copyright
© 2020 The Authors. ChemBioChem published by Wiley-VCH GmbH.
Références
Annu Rev Biophys. 2016 Jul 5;45:1-23
pubmed: 27145877
Photochem Photobiol Sci. 2010 Dec;9(12):1634-9
pubmed: 20931137
Methods. 2007 Feb;41(2):158-67
pubmed: 17189858
Chembiochem. 2021 Feb 2;22(3):548-556
pubmed: 32974998
Chem Commun (Camb). 2012 May 14;48(38):4603-5
pubmed: 22466119
Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7
pubmed: 25713381
Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4756-61
pubmed: 22411832
J Mol Biol. 1987 Sep 20;197(2):257-71
pubmed: 2445998
Nucleic Acids Res. 2012 Dec;40(22):e176
pubmed: 22923521
Nucleic Acids Res. 2007;35(16):e107
pubmed: 17704126
Biochem Soc Trans. 2018 Jun 19;46(3):729-740
pubmed: 29871877
Chem Sci. 2018 Dec 12;10(7):1962-1970
pubmed: 30881625
Chem Commun (Camb). 2014 Jun 11;50(46):6149-52
pubmed: 24776726
Nat Methods. 2018 May;15(5):347-350
pubmed: 29578537
Mutat Res. 1987 Oct;182(5):265-74
pubmed: 3309639
Nat Genet. 2020 Jan;52(1):29-34
pubmed: 31844324
J Am Chem Soc. 2017 Oct 25;139(42):15168-15175
pubmed: 29039200
Cell. 2013 Dec 19;155(7):1479-91
pubmed: 24360272
Arzneimittelforschung. 1974 Dec;24(12):1927-33
pubmed: 4480273
Nat Rev Mol Cell Biol. 2017 Nov;18(11):685-701
pubmed: 28875992
Photochem Photobiol Sci. 2002 Apr;1(4):225-36
pubmed: 12661961
EMBO J. 2009 Dec 16;28(24):3785-98
pubmed: 19927119
Nat Biotechnol. 2016 May;34(5):528-30
pubmed: 27088723
Nat Rev Microbiol. 2017 Feb;15(2):69-82
pubmed: 27941817
Chem Commun (Camb). 2014 Aug 21;50(65):9141-4
pubmed: 24989898
Org Lett. 2019 Dec 6;21(23):9410-9414
pubmed: 31714093
ACS Chem Biol. 2018 Oct 19;13(10):2888-2896
pubmed: 30111097
Comput Struct Biotechnol J. 2019 Nov 30;18:27-34
pubmed: 31890141
J Cell Biol. 2014 May 26;205(4):591-606
pubmed: 24862576
J Cell Biol. 2010 Nov 15;191(4):875-90
pubmed: 21059852
J Histochem Cytochem. 1982 Feb;30(2):111-6
pubmed: 7061816
Nature. 1981 Apr 9;290(5806):527-8
pubmed: 7219539
Nat Commun. 2015 Oct 01;6:8497
pubmed: 26423723
Nat Commun. 2017 Mar 14;8:14725
pubmed: 28290446
Cytometry A. 2005 Sep;67(1):45-52
pubmed: 16082711
Chem Rev. 2013 Jan 9;113(1):119-91
pubmed: 23256727