Human Retinal Microvasculature-on-a-Chip for Drug Discovery.
3D models
blood-retinal barriers
microvasculatures
organ-on-a-chip
Journal
Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
28
08
2020
pubmed:
26
9
2020
medline:
15
5
2021
entrez:
25
9
2020
Statut:
ppublish
Résumé
Retinal cells within neurovascular units generate the blood-retinal barrier (BRB) to regulate the local retinal microenvironment and to limit access to inflammatory cells. Breakdown of the endothelial junctional complexes in the BRB negatively affects neuronal signaling and ultimately causes vision loss. As new therapeutics are being developed either to prevent barrier disruption or to restore barrier function, access to physiologically relevant human in vitro tissue models that recapitulate important features of barrier biology is essential for disease modeling, target validation, and toxicity assessment. Here, a tunable organ-on-a-chip model of the retinal microvasculature using human retinal microvascular endothelial cells with integrated flow is described. Automated imaging and image analysis methods are employed for facile screening of leakage mediators and cytokine inhibitors on barrier properties. The developed retinal microvasculature-on-a-chip will enable improved understanding of BRB biology and provide an additional tool for drug discovery.
Identifiants
pubmed: 32975047
doi: 10.1002/adhm.202001531
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2001531Informations de copyright
© 2020 Wiley-VCH GmbH.
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