Hyperosmolar Ionic Solutions Modulate Inflammatory Phenotype and sGAG Loss in a Cartilage Explant Model.
cartilage explant
hyperosmolar solution
inflammation
lithium
osteoarthritis
potassium
sodium
Journal
Cartilage
ISSN: 1947-6043
Titre abrégé: Cartilage
Pays: United States
ID NLM: 101518378
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
pubmed:
26
9
2020
medline:
7
4
2022
entrez:
25
9
2020
Statut:
ppublish
Résumé
The objective of this study was to compare the effects of hyperosmolar sodium (Na Explants from bovine stifle joints were stimulated with TNF-α for 1 day to induce cartilage degradation followed by supplementation with 50 mM potassium chloride (KCl), 50 mM lithium chloride (LiCl), 50 mM sodium chloride (NaCl), or 100 nM dexamethasone for an additional 6 days. We assessed the effect of TNF-α stimulation and hyperosmolar ionic treatment on sGAG loss and expression of OA-associated proteins: ADAMTS-5, COX-2, MMP-1, MMP-13, and VEGF. TNF-α treatment increased sGAG loss ( These results suggest that these ionic species elicit varying responses in TNF-α-stimulated explants. Cumulatively, these findings support additional studies of hyperosmolar ionic solutions for potential development of novel intraarticular injections targeting OA.
Identifiants
pubmed: 32975437
doi: 10.1177/1947603520961167
pmc: PMC8804856
doi:
Substances chimiques
Glycosaminoglycans
0
A73025
268AW7000T
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
713S-721SSubventions
Organisme : NIA NIH HHS
ID : R03 AG056168
Pays : United States
Organisme : NIA NIH HHS
ID : R03 AG064550
Pays : United States
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