Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury.
Animals
Disease Models, Animal
Electrophysiology
G Protein-Coupled Inwardly-Rectifying Potassium Channels
/ analysis
Ganglia, Spinal
/ injuries
Hyperalgesia
Male
Membrane Potentials
/ drug effects
Mice
Mice, Inbred C57BL
Neuralgia
Patch-Clamp Techniques
/ methods
Peripheral Nerve Injuries
/ metabolism
Sciatic Nerve
/ injuries
Sensory Receptor Cells
/ physiology
Chronic constriction injury
Dorsal root ganglion neurons
GIRK currents
Kir currents
Neuropathic pain
Opioid receptors
Opioids
Potassium channels
Whole-cell patch clamp
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
25
9
2020
pubmed:
26
9
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
Patch clamp is an electrophysiological technique that allows to analyze the activity of ion channels in neurons. In this chapter, we provide a detailed description of patch clamp protocol to measure the effect of a μ-opioid receptor agonist on the activity of G protein-coupled inwardly rectifying potassium (GIRK or Kir3) channels. This is performed in peripheral sensory neurons isolated from dorsal root ganglia (DRG) of mice without or with a chronic constriction injury (CCI) of the sciatic nerve, which models neuropathic pain. We describe the induction of the CCI , isolation and culture of DRG neurons, performance of the patch clamp recordings, and identification of opioid-responding neurons.
Identifiants
pubmed: 32975795
doi: 10.1007/978-1-0716-0884-5_12
doi:
Substances chimiques
G Protein-Coupled Inwardly-Rectifying Potassium Channels
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM