Rescue of SARS-CoV-2 from a Single Bacterial Artificial Chromosome.
Animals
Betacoronavirus
/ genetics
COVID-19
Chlorocebus aethiops
Chromosomes, Artificial, Bacterial
/ genetics
Coronavirus Infections
/ virology
Cricetinae
DNA, Complementary
/ genetics
Genome, Viral
/ genetics
Pandemics
Pneumonia, Viral
/ virology
RNA, Viral
/ genetics
Reverse Genetics
SARS-CoV-2
Vero Cells
Virus Replication
BAC
COVID-19
SARS-CoV-2
coronavirus
hamsters
recombinant virus
reverse genetics
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
25 09 2020
25 09 2020
Historique:
entrez:
26
9
2020
pubmed:
27
9
2020
medline:
21
10
2020
Statut:
epublish
Résumé
Infectious coronavirus (CoV) disease 2019 (COVID-19) emerged in the city of Wuhan (China) in December 2019, causing a pandemic that has dramatically impacted public health and socioeconomic activities worldwide. A previously unknown coronavirus, severe acute respiratory syndrome CoV-2 (SARS-CoV-2), has been identified as the causative agent of COVID-19. To date, there are no U.S. Food and Drug Administration (FDA)-approved vaccines or therapeutics available for the prevention or treatment of SARS-CoV-2 infection and/or associated COVID-19 disease, which has triggered a large influx of scientific efforts to develop countermeasures to control SARS-CoV-2 spread. To contribute to these efforts, we have developed an infectious cDNA clone of the SARS-CoV-2 USA-WA1/2020 strain based on the use of a bacterial artificial chromosome (BAC). Recombinant SARS-CoV-2 (rSARS-CoV-2) was readily rescued by transfection of the BAC into Vero E6 cells. Importantly, BAC-derived rSARS-CoV-2 exhibited growth properties and plaque sizes in cultured cells comparable to those of the natural SARS-CoV-2 isolate. Likewise, rSARS-CoV-2 showed levels of replication similar to those of the natural isolate in nasal turbinates and lungs of infected golden Syrian hamsters. This is, to our knowledge, the first BAC-based reverse genetics system for the generation of infectious rSARS-CoV-2 that displays features
Identifiants
pubmed: 32978313
pii: mBio.02168-20
doi: 10.1128/mBio.02168-20
pmc: PMC7520601
pii:
doi:
Substances chimiques
DNA, Complementary
0
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2020 Ye et al.
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