Heterogeneous ozone effects on the DNA methylome of bronchial cells observed in a crossover study.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 09 2020
25 09 2020
Historique:
received:
09
01
2020
accepted:
18
08
2020
entrez:
26
9
2020
pubmed:
27
9
2020
medline:
13
1
2021
Statut:
epublish
Résumé
We used a randomized crossover experiment to estimate the effects of ozone (vs. clean air) exposure on genome-wide DNA methylation of target bronchial epithelial cells, using 17 volunteers, each randomly exposed on two separated occasions to clean air or 0.3-ppm ozone for two hours. Twenty-four hours after exposure, participants underwent bronchoscopy to collect epithelial cells whose DNA methylation was measured using the Illumina 450 K platform. We performed global and regional tests examining the ozone versus clean air effect on the DNA methylome and calculated Fisher-exact p-values for a series of univariate tests. We found little evidence of an overall effect of ozone on the DNA methylome but some suggestive changes in PLSCR1, HCAR1, and LINC00336 DNA methylation after ozone exposure relative to clean air. We observed some participant-to-participant heterogeneity in ozone responses.
Identifiants
pubmed: 32978449
doi: 10.1038/s41598-020-72068-6
pii: 10.1038/s41598-020-72068-6
pmc: PMC7519112
doi:
Substances chimiques
HCAR1 protein, human
0
PLSCR1 protein, human
0
Phospholipid Transfer Proteins
0
RNA, Long Noncoding
0
Receptors, G-Protein-Coupled
0
Ozone
66H7ZZK23N
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
15739Subventions
Organisme : NIH HHS
ID : DP5OD021412
Pays : United States
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