Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization.
Dedifferentiation
Melanoblasts
Melanocytes
Trypsinization
Vitiligo
Journal
Cell and tissue banking
ISSN: 1573-6814
Titre abrégé: Cell Tissue Bank
Pays: Netherlands
ID NLM: 100965121
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
06
06
2020
accepted:
18
09
2020
revised:
26
07
2020
pubmed:
27
9
2020
medline:
16
11
2021
entrez:
26
9
2020
Statut:
ppublish
Résumé
Human epidermal melanocytes can be induced to form melanocyte spheroids and revert to immature characteristics by long-term trypsinization (LTT). To further explore the biological characteristics of melanocytes after LTT and to study the underlying mechanism. Melanocytes were subjected to long-term (2 h) trypsinization in this study, after which their viability, proliferation and autophagy were characterized. The expression of melanocyte markers [human melanoma black45 (HMB45), tyrosinase (TYR) and Nestin] was detected and relative expression levels of mRNAs encoding TYR, Nestin, c-Kit and microphthalmia-associated transcription factor (MITF) were determined. After LTT, more short spindle-shaped melanocytes appeared and viability assays demonstrated that most melanocytes survived that treatment but had decreased proliferation rates compared to the untreated controls. There was a significant increase in autophagy of melanocytes after LTT and the expression of TYR was decreased compared with untreated control melanocytes. There were no significant differences in the expression of HMB45 or Nestin between the two groups. Compared with untreated melanocytes, levels of message ribonucleic acid (mRNAs) encoding TYR, c-Kit and MITF were decreased after LTT, while Nestin mRNA levels were increased. These results clarified that Long-term treatment with trypsin causes the dedifferentiation of mature epidermal melanocytes in vitro.
Identifiants
pubmed: 32978700
doi: 10.1007/s10561-020-09866-9
pii: 10.1007/s10561-020-09866-9
doi:
Substances chimiques
RNA, Messenger
0
Monophenol Monooxygenase
EC 1.14.18.1
Proto-Oncogene Proteins c-kit
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
67-75Subventions
Organisme : National Natural Science Foundation of China
ID : 81673078
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