Plasmodium berghei sporozoites in nonreplicative vacuole are eliminated by a PI3P-mediated autophagy-independent pathway.
Animals
Autophagy
Cell Line
Female
HeLa Cells
Hepatocytes
/ parasitology
Host-Parasite Interactions
Humans
Malaria
/ parasitology
Mice
Microtubule-Associated Proteins
/ metabolism
Organisms, Genetically Modified
Phosphatidylinositol Phosphates
/ metabolism
Plasmodium berghei
/ metabolism
Sporozoites
/ metabolism
Vacuoles
/ parasitology
Plasmodium
autophagy
elimination
liver stage
transient vacuole
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
04
06
2020
revised:
17
09
2020
accepted:
18
09
2020
pubmed:
27
9
2020
medline:
21
10
2021
entrez:
26
9
2020
Statut:
ppublish
Résumé
The protozoan parasite Plasmodium, causative agent of malaria, invades hepatocytes by invaginating the host cell plasma membrane and forming a parasitophorous vacuole membrane (PVM). Surrounded by this PVM, the parasite undergoes extensive replication. Parasites inside a PVM provoke the Plasmodium-associated autophagy-related (PAAR) response. This is characterised by a long-lasting association of the autophagy marker protein LC3 with the PVM, which is not preceded by phosphatidylinositol 3-phosphate (PI3P)-labelling. Prior to productive invasion, sporozoites transmigrate several cells and here we describe that a proportion of traversing sporozoites become trapped in a transient traversal vacuole, provoking a host cell response that clearly differs from the PAAR response. These trapped sporozoites provoke PI3P-labelling of the surrounding vacuolar membrane immediately after cell entry, followed by transient LC3-labelling and elimination of the parasite by lysosomal acidification. Our data suggest that this PI3P response is not only restricted to sporozoites trapped during transmigration but also affects invaded parasites residing in a compromised vacuole. Thus, host cells can employ a pathway distinct from the previously described PAAR response to efficiently recognise and eliminate Plasmodium parasites.
Identifiants
pubmed: 32979009
doi: 10.1111/cmi.13271
pmc: PMC7757174
doi:
Substances chimiques
MAP1LC3A protein, human
0
Microtubule-Associated Proteins
0
Phosphatidylinositol Phosphates
0
phosphatidylinositol 3-phosphate
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13271Informations de copyright
© 2020 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
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