Effects of optogenetic activation of the enteric nervous system on gastrointestinal motility in mouse small intestine.
Autonomic nervous system
Colon
Enteric nervous system
Motility
Peristalsis
Sensory neuron
Journal
Autonomic neuroscience : basic & clinical
ISSN: 1872-7484
Titre abrégé: Auton Neurosci
Pays: Netherlands
ID NLM: 100909359
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
05
04
2020
revised:
17
08
2020
accepted:
16
09
2020
pubmed:
28
9
2020
medline:
6
10
2021
entrez:
27
9
2020
Statut:
ppublish
Résumé
Recently, it was demonstrated that optogenetics could be used to stimulate enteric calretinin neurons, leading to increased colonic transit in vitro and in vivo. The aim of the current study was to determine if similar approaches could be used to stimulate the isolated mouse small intestine, with the aim of potentially also improving transit in the small bowel. Cre-Lox recombination was used to generate transgenic mice expressing the light-sensitive ion channel channelrhodopsin-2 (ChR2) in calretinin neurons. Spontaneous migrating motor complexes were recorded from isolated terminal small intestine in both Cal Focal illumination of the small intestine does not appear as effective at inducing propulsive motor activity as has been demonstrated in the colon of the same colony mice. This study suggests caution should be exercised when assuming optogenetic technology is equally effective at increasing GI transit in the small intestine as in the large intestine of mice.
Sections du résumé
BACKGROUND AND AIMS
Recently, it was demonstrated that optogenetics could be used to stimulate enteric calretinin neurons, leading to increased colonic transit in vitro and in vivo. The aim of the current study was to determine if similar approaches could be used to stimulate the isolated mouse small intestine, with the aim of potentially also improving transit in the small bowel.
METHODS
Cre-Lox recombination was used to generate transgenic mice expressing the light-sensitive ion channel channelrhodopsin-2 (ChR2) in calretinin neurons.
RESULTS
Spontaneous migrating motor complexes were recorded from isolated terminal small intestine in both Cal
CONCLUSION
Focal illumination of the small intestine does not appear as effective at inducing propulsive motor activity as has been demonstrated in the colon of the same colony mice. This study suggests caution should be exercised when assuming optogenetic technology is equally effective at increasing GI transit in the small intestine as in the large intestine of mice.
Identifiants
pubmed: 32980660
pii: S1566-0702(20)30167-3
doi: 10.1016/j.autneu.2020.102733
pmc: PMC9884517
mid: NIHMS1864619
pii:
doi:
Substances chimiques
Calb2 protein, mouse
0
Calbindin 2
0
Channelrhodopsins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102733Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK052574
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK103901
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM101218
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
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